FIBROBLAST growth factorsBackground: Human dermal fibroblasts secrete numerous growth factors and proteins that have been suggested to promote wound repair and hair regeneration. Methods: Human dermal fibroblast-conditioned medium (DFCM) was prepared, and proteomic analysis was performed. ...
We have also recently observed that the cytokine CSF-1 is both present on the surface of EVs and as a soluble form in the conditioned medium of some tumor cell lines, and that EV-associated CSF1 and recombinant CSF1 induce different gene expression signatures in monocytes54. In sum, the ...
Within the gastrointestinal tract, FGF-7, or keratinocyte growth factor (KGF) and its corresponding receptor, FGFR2-IIIb, have been extensively studied.1048–1051 KGF was originally isolated from the conditioned medium of human lung fibroblasts and characterized as a potent mitogen for keratinocytes....
The medium was collected and centrifuged at 135g for 3 min to remove cell debris. The conditioned medium, thus collected, was then utilized to treat pancreatic cancer cell lines or stored at −80 °C until use. Cell viability assays Cell viability was determined by 3-[4,5-...
Within the gastrointestinal tract, FGF-7, orkeratinocytegrowth factor (KGF) and its corresponding receptor, FGFR2-IIIb, have been extensively studied (1048–1051). KGF was originally isolated from the conditioned medium of humanlung fibroblastsand characterized as a potentmitogenforkeratinocytes(1052)....
Pirfenidone, an antifibrotic agent used for the treatment of idiopathic pulmonary fibrosis (IPF), functions by inhibiting myofibroblast differentiation, which is involved in transforming growth factor (TGF)-β1-induced IPF pathogenesis. However, unlike n
Cancer-associated fibroblasts (CAFs) play a key role in cancer progression and treatment outcome. This study dissects the intra-tumoral diversity of CAFs in basal cell carcinoma, squamous cell carcinoma, and melanoma using molecular and spatial single-ce
HIMF expression in cardiac fibroblasts cannot be stimulated by transverse aortic constriction or exposure to prohypertrophic factors, including phenylephrine, Ang II (angiotensin II), TGF (transform growth factor)-beta, and hypoxia. However, conditioned medium from cardiomyocytes overexpressing HIMF can ...
with CD248ECD-EGFP. Black and gray lines indicate results for WT andLgals3–/–BMDMs, respectively, with control medium. (e) Flow cytometry of CD248ECD-EGFP binding to WT BMDMs in the presence of 25 mM lactose (red) or sucrose (blue). Conditioned medium from HEK293T cells in the pres...
wherein the cells whose growth is to be promoted are chosen from the group consisting of cells in the vicinity of a wound, cells in the vascular system, cells involved in hematopoiesis, cells involved in erythropoiesis, cells in the lining of the gastrointestinal tract, and cells in hair ...