SourcePolyclonal antibody preparation HostRabbit Potencyn/a Ig TypeIgG PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography LabelNone ImmunogenRPA705Hu01-Recombinant Mucin 2 (MUC2) Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol....
levels were comparable between mucin TR reporters and between isogenic cell lines as confirmed by anti-FLAG staining (Supplementary Fig.8a). Thus, we found that the selective binding of HsaBRto MUC2#1, MUC7, and GP1bα was unaffected by limiting O-glycans to mSTa (KOST6GALNACs), while bind...
4E). The vacuoles were positive for MUC2, specific for goblet cells in the small intestine (Fig. 4F). Electron microscopy analysis clearly revealed microvilli lined with actin filaments and a glycocalyx on the apical membrane (Figs. 4G and 4H). Intestinal epithelial cells are composed of ...
markerVILLIN(VIL1) gene, goblet cell markerMUCIN(MUC2) gene, Paneth cell markerlysozyme(LYZ) gene expressions in differentiated cells at day 35–46. Data are presented as means ± S.D. Fold changes versus that of day 3 definitive endodermal cells are shown (differentiated cells at day 3: ...
(Sakura Finetek), whereas antibody stains were performed using a Ventana Benchmark ULTRA (Roche) or Dako Omnis (Agilent Technologies) automated solutions. Slides of human appendix (CK20, MUC2 and CHGA) or humantonsils(Ki67) were used as positive antibody controls and were stained simultaneously ...
(NPs), enhances its stability such that it now supports organ stem cells in the absence of serum and CHAPS. Moreover, we show that the affinity of Wnt3a to lipids has applications in the purification of recombinant Wnt3a. Our findings constitute an important step towards the use of human ...
Fecal-adherent mucus is a non-invasive source of primary human MUC2 for structural and functional characterization in health and disease The O-glycoprotein Mucin-2 (MUC2) forms the protective colon mucus layer. While animal models have demonstrated the importance of Muc2, few studies have ex... ...
The intestinal MUC2 mucin C-terminus is stabilized by an extra disulfide bond in comparison to von Willebrand factor and other gel-forming mucins Article Open access 08 April 2023 Introduction Mucins comprise a family of 21 high molecular weight secretory or membrane-bound glycoproteins that provide...
The present invention relates to novel antibodies and fragments thereof that binds cannabinoid 1 (CB1) receptor. The antibodies and fragments thereof as disclosed herein include hum
O-Glycosylated MUC2 Monomer and Dimer from LS 174T Cells Are Water-soluble, whereas Larger MUC2 Species Formed Early during Biosynthesis Are Insoluble and ... The MUC2 mucin is the major gel-forming mucin in the small and large intestine. Due to its sequence similarities with the von Willebran...