The antibody is a rabbit polyclonal antibody raised against MUC2. It has been selected for its ability to recognize MUC2 in immunohistochemical staining and western blotting. USAGEWestern blotting: 0.01-2µg/mL;Immunohistochemistry: 5-20µg/mL;Immunocytochemistry: 5-20µg/mL;Optimal working ...
function and/or maturation impairment, both suggested by the gene expression data set observed in organoids (Fig.1c–e). Consistent with the gene expression data the immunofluorescence staining of the ZO1 showed less deposition of this protein along the cell perimeter of CD monolayers (Fig.3d),...
F Immunohistochemistry of RYU in iPGell with antibodies to MUC2. Scale bar: 50 µm. G Transmission electron microscopic analysis of epithelial apical membrane side. Microvilli and glycocalyx (yellow squares) were observed. Scale bar: 200 nm. H Scanning electron microscopic analysis of RYU on ...
Next, we tested the ability of Pic to degrade the MUC2 layer by incubating 75C colonoids with 1X PBS or 1 μM of purified Pic for 6 h at 37 °C. Subsequently, colonoid monolayers were stained for MUC2 and for cell nuclei, and analyzed by confocal microscopy (Fig.2a). MUC2 v...
The main component of both layers is the highly glycosylated Mucin 2 (MUC2) which is produced, stored and secreted by goblet cells [1]. Increased mucus secretion in the crypts is a response to stress stimuli such as bacterial invasion [2, 3]. The elevated secretion facilitates the ...
To visualize enteroid cell differentiation into the various IEC lineages, we performed confocal immunofluorescence microscopy. We show that cells in apical-out enteroids in differentiation media express markers for Paneth cells (lysozyme), goblet cells (MUC2), entero-endocrine cells (chromogranin A [CH...
The tumor cells retained the transduced GFP-linked constructs, as determined via immunofluorescence on tumor sections (Fig. 6C). Neither over-expression of the CaSR, nor its activation with NPS R-568 affected tumor angiogenesis, as shown by staining with a specific antibody against murine platelet...
Anti-Muc2 粘蛋白-2/上皮膜抗原2抗体Multi-class antibodies规格: 0.2ml Rabbit anti-Homo sapiens (Human) COX6B1 Polyclonal 抗体抑制素β-B(Inhbb)ELISA试剂盒 ,英文名: Inhbb ELISA Kit Anti-PAX8 配对盒基因8抗体Multi-class antibodies规格: 0.2ml 大鼠L选择素(L-Selectin/CD62L)免疫试剂盒 Rat L-Select...
PDO11 also showed an abundant expression of the goblet cell-specific marker MUC2 and an active epigenetic state across the gene (Supplementary Fig. 2e), suggesting a mucinous phenotype that is consistent with the MSI status of this tumor19. This de novo chromatin state characterization provided a...
Immunofluorescence staining for MUC2 showed characteristic rimming of cells throughout the structures (Figure 4c). CK20 expression was limited to the interior of the structures (Figure 4c, inset). Immunoperoxidase stained cross-sections of paraffin- embedded adenoma structure (day 73) show that MUC2...