If the antibody is used in flow cytometry, please check FCM antibodies. DownloadInstruction Manual UOM 20µl100µl 200µl 1ml 10ml FOB US$ 221 For more details, please contact local distributors!SPECIFITYThe antibody is a rabbit polyclonal antibody raised against MUC2. It has been ...
StcEE447D, X409 domain truncated StcE (StcEΔX409), and the isolated X409 domains ± GFP (left). Flow cytometry analysis of cleavage (dose titration) of membrane-bound MUC2#1 expressed in HEK293WTcells by StcEWT, Stc
PDO11 also showed an abundant expression of the goblet cell-specific marker MUC2 and an active epigenetic state across the gene (Supplementary Fig. 2e), suggesting a mucinous phenotype that is consistent with the MSI status of this tumor19. This de novo chromatin state characterization provided a...
To determine a potential role for human secreted mucins in the modulation of DC function, human moDCs were treated with purified mucin MUC2. The phenotype of human moDCs was confirmed by flow cytometry, with cells showing a lack of CD14 expression and reduced expression of CD33 compared with ...
(EPCAM-positive) shared between fetal, paediatric and adult datasets were stem cells (LGR5, ASCL2, SMOC2, RGMB, OLFM4), Paneth (DEFA5, DEFA6, REG3A), transit-amplifying (TA; MKI67, TOP2A, PCNA), goblet cells (CLCA1, SPDEF, FCGBP, ZG16, MUC2), BEST4 enterocytes (BEST4, OTOP2...
added to the wells at day 0 only and the cells were no longer refreshed. The ESCs were imaged every day with an Olympus IX-70 inverted fluorescent microscope. Following imaging, one well of ESCs from each condition was analysed for GFP expression by flow cytometry (Fortessa, BD biosciences)...
Similarly, mucin-related genes (MUC20, MUC2, MUC17, MUC4, MUC12, MUC3A, MUC1, and MUC6) were also higher in postnatal cells, suggesting that the mucin layer covering the intestinal epithelium develops postnatally (Supplementary Fig. 2H and Supplementary Table 5; epithelial cells). Moreover,...
(f), MUC2 (g) and CHGA (h) evaluated the relative abundance of Paneth’s cells, enterocytes, goblet’s cells and neuroendocrine cells, respectively. Data represent average expression relative to NC non-differentiated ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, two-...
For example, mice lacking Muc2 and secreted mucus develop colitis but no pathology in the ileum due to the lower abundance of bacteria in the small intestine and induction of the IL-22 pathway of genes regulating barrier function and innate defences23. Analysis of the colon tissue gene ...
(C) The proportion of EpCAM+cells in pre-separation cells before MACS, positive fraction after MACS and negative fraction after MACS are analyzed by flow cytometry. (D) Representative immuno-staining images of VILLIN, LYZ, MUC2, CHGA, and EphB2 in EpCAM+cells (from the left), and bright...