Pearson correlation between log10(x + 1)-transformed observed mRNA counts and expected mRNA amount from the cell2location model assessed the model quality (Pearson R = 0.745). Micro-anatomical tissue environments were labeled from the H&E images. Only Visium spots aligned and annotated ...
resulting in early-onset epilepsy, developmental delay, cognitive disabilities, and cerebellar atrophy. Patient lymphoblasts show impaired cell survival, proliferation, and hypersensitivity to oxidative stress. These phenotypes are rescued by TLDc domain replacement. We generate patient-derived induced pluripo...
Single-cell transcriptomics of a human kidney allograft biopsy specimen defines a diverse inflammatory response. J. Am. Soc. Nephrol. 29, 2069–2080 (2018). Article CAS PubMed PubMed Central Google Scholar Kim, N. et al. Single-cell RNA sequencing demonstrates the molecular and cellular ...
Breitholtz-Emanuelsson, A., Minervini, F., Hult, K., Visconti, A., 1994: Ochratoxin A in human serum samples collected in southern Italy from healthy individuals and individuals suffering from different kidney disorders. Natural Toxins 2, 366–370 CAS Google Scholar Studer-Rohr, I., Schlatt...
aDetection of HRH2 in Huh7.5.1difcells by immunofluorescence. HRH2 is shown in magenta (Alexa FluorTM647) and nuclei in blue (DAPI). CTRL = cells incubated with AF647-labeled secondary antibody. Scale bar: 10 µm.bPLS assessment upon perturbation of the HRH2/cAMP/PKA axis. PLS...
The human keratin 18 promoter was used to overexpress hACE2 receptor in the epithelial cells of the most commonly used K18-hACE2-B6 model. Specifically, over-expression of human ACE2 was identified in airway epithelia and the epithelia lining the liver, kidney and gastrointestinal tract in K18...
HEK human embryonic kidney Ig immunoglobulin IgG immunoglobulin G LC light chain RB Russell body VL light chain variable domain VH heavy chain variable domain Keywords Russell body Immunoglobulin Protein aggregation Endoplasmic reticulum Recombinant antibody Protein folding ...
To generate organoids compatible with HTS, hPSCs were plated in 96- and 384-well formats and differentiated into the kidney lineage for 3 weeks (Figure 1A). Kidney organoids are highly complex and of great biomedical interest for their potential to model disease, toxicity, and regeneration (Fre...
Defining cellular and molecular identities within the kidney is necessary to understand its organization and function in health and disease. Here we demonstrate a reproducible method with minimal artifacts for single-nucleus Droplet-based RNA sequencing
We have successfully cleared multiple human organs, including kidney, pancreas, heart, lung, spleen and brain, as well as hard tissue like skull. We also describe an advanced volumetric imaging system using a commercial light-sheet fluorescence microscope that can accommodate most human organs and ...