Non-unique sgRNAs in GeCKOv2 human CRISPR libraryNeville, Sanjana
Arrayed CRISPR libraries extend the scope of gene-perturbation screens to non-selectable cell phenotypes. However, library generation requires assembling thousands of vectors expressing single-guide RNAs (sgRNAs). Here, by leveraging massively parallel p
The early genome-scale CRISPR knockout library version 2 (GeCKOv2) library contained sgRNAs targeting miRNAs [19], and a selectively miRNA-targeting CRISPR-Cas9 library has been described [20]. To facilitate the screening of miRNA function in human cells, we designed a miRNA-targeting CRISPR-...
Genome-wide functional screening using the CRISPR-Cas9 system is a powerful tool to uncover tumor-specific and common genetic dependencies across cancer cell lines. Current CRISPR-Cas9 knockout libraries, however, primarily target protein-coding genes. T
For GeCKOv2, we used the cell lines that had exactly 4 replicates (29 of 33). Rights and permissions Reprints and permissions About this article Cite this article DeWeirdt, P.C., Sanson, K.R., Sangree, A.K. et al. Optimization of AsCas12a for combinatorial genetic screens in human ...
42 Although some of these approaches have entered the clinic, concerns have been raised about undesirable impacts of the double-strand DNA breaks produced by CRISPR-Cas9.2,3,4,5,6 A more controlled and potentially safer approach to abrogate gene function might be to use base editors to alter...
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Genome-wide clustered regularly interspaced short palindromic repeats (CRISPR)-based knockout screening is revolting the genetic analysis of a cellular or molecular phenotype in question but is challenged by the large size of single-guide RNA (sgRNA) library. Here we designed a minimal genome-wide ...
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