We show that lentiviral delivery of a genome-scale CRISPR-Cas9 knockout (GeCKO) library targeting 18,080 genes with 64,751 unique guide sequences enables both negative and positive selection screening in human cells. First, we used the GeCKO library to identify genes essential for...
The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimenta...
A genome-wide CRISPR-Cas9 knockout screen identifies hTSC EGs We transduced the blastocyst-derived BT5 hTSC line8with the Brunello human CRISPR knockout pooled lentiviral library, which contains 76,441 single guide RNAs (sgRNAs) targeting 19,114 genes23. Following puromycin selection, the cells w...
Genome-wide functional screening using the CRISPR-Cas9 system is a powerful tool to uncover tumor-specific and common genetic dependencies across cancer cell lines. Current CRISPR-Cas9 knockout libraries, however, primarily target protein-coding genes. T
et al. In vivo profiling of metastatic double knockouts through CRISPR-Cpf1 screens. Nat. Methods 16, 405–408 (2019). Article CAS PubMed PubMed Central Google Scholar Liu, J. et al. Pooled library screening with multiplexed Cpf1 library. Nat. Commun. 10, 3144 (2019). Article PubMed...
Genome-wide functional screening using the CRISPR-Cas9 system is a powerful tool to uncover tumor-specific and common genetic dependencies across cancer cell lines. Current CRISPR-Cas9 knockout libraries, however, primarily target protein-coding genes. T
Synonyms:Human CRISPR-sgRNA knockout dual plasmid mixed library B (communication with technology) CAS: MF: MW:0 EINECS: Mol File:Mol File Information Error Report Your Email: Browse by NationalityHuman CRISPR-sgRNA knockout dual plasmid mixed library B (communication with technology)SuppliersChina ...
世联博研公司现货供应ORIGENE TECHNOLOGIES品牌订货号为KN401484的RIOK2 - KN2.0, Human gene knockout kit via CRISPR from ORIGENE TECHNOLOGIES产品
世联博研公司现货供应ORIGENE TECHNOLOGIES品牌订货号为KN419123的NANOS1 - KN2.0, Human gene knockout kit via CRISPR from ORIGENE TECHNOLOGIES产品
Genome-wide clustered regularly interspaced short palindromic repeats (CRISPR)-based knockout screening is revolting the genetic analysis of a cellular or molecular phenotype in question but is challenged by the large size of single-guide RNA (sgRNA) library. Here we designed a minimal genome-wide ...