The exosomes were isolated from the culture medium of human ESCC cells (TE2, T.Tn) by ultracentrifugation, and cell proliferation assay, wound healing assay, and fluorescence imaging of the cell cycle were performed to clarify the phenotypic changes in the high concentration of tumor-derived ...
TRAP1 was overexpressed in ESCC, particularly in poorly differentiated tumors. To further explore the molecular regulatory mechanism, we constructed specific small interfering RNA-expressing vectors targeting Trap1, and knocked down Trap1 expression in the esophageal cancer cell lines ECA109 and EC9706....
Two human ESCC cell lines, TE-13 and Eca109 were studied. Silencing of cell line HO-1 by specific small interfering RNA (siRNA) was evaluated using real-time quantitative PCR. Cell line viability, apoptosis and intracellular levels of reactive oxygen species (ROS) after transfection were ...
Methods We examined whether fascin is a potential target in ESCC using in vitro and in vivo studies utilizing a specific siRNA. We established a stable transfectant with downregulated fascin from KYSE170 cell line. Results The fascin downregulated cell lines showed a slower growth pattern by 40.3...
Fascin overexpression was associated with poor prognosis, and its downregulation reduces cell motility and invasiveness in esophageal squamous cell carcinoma (ESCC). Using a stable knockdown cell line, we revealed the effect of fascin on cell growth, cell adhesion and tumor formation. Methods We ...
Fig. 1: Profile of immune infiltrates in human ESCC with scRNA-seq and scTCR-seq. aSchematic diagram of the experimental design and analysis.bUMAP plot of 80,787 high-quality immune cells to visualize cell-type clusters based on the expression of known marker genes.cHeatmap of the relative...
Permanent cancer cell lines are invaluable tools in understanding the biology of cancers and experimental therapeutics. To enrich cell line panel and animal models of human esophageal squamous cell carcinoma (ESCC) from different geographical areas and investigate the environmental and genetic risk factors...
[61]. The high levels of Fstl1 correlate with lower survival perspective in HNSCC and ESCC patients [62,63]. Most cell lines derived from human tumours express lower levels of FSTL1 than immortalized non-tumourigenic fibroblasts [65]. In vitro transfection of fibroblasts (mouse NIH3T3 and ra...
ability of cancer cells compared to control cells [31]. Therefore, these researchers concluded that circ_0067934 contributes to cancer cell motility and migration, which may affect cell cycle status and become a new therapeutic target for ESCC. Elevated circ_0067934 expression was also reported to ...
The enhancement of radiosensitivity in human esophageal squamous cell carcinoma cells by zoledronic acid and its potential mechanism Esophageal squamous cell carcinoma (ESCC) has a low 5-year patient survival rate. Radiotherapy, as a preoperative or postoperative treatment of surgery, ha... Y You,J...