Methods: Human embryonic stem cell lines (Royan H1) were used to produce embryoid bodies. Differentiation carried out by growth factor-mediated selection of nestin positive cells. In final stage, these cells were expanded in the presence of bFGF, followed by addition of nicotinamide to promote ...
The potential of human embryonic stem (hES) cells to differentiate into cell types of a variety of organs has generated much excitement over the possible use of hES cells in therapeutic applications. Of great interest are organs derived from definitive e
Using a sequence of growth-factor manipulations to mimic embryonic intestinal development in culture, a new study has successfully generated human intestinal tissue in vitro from embryonic and induced pluripotent stem cells. The resulting epithelium was
Human embryonic stem cells (hESCs) have been extensively discussed in public and scientific communities for their potential in treating diseases and injuries. However, not much has been achieved in turning them into safe therapeutic agents. The hurdles i
Human embryonic stem cells (hESCs) have extensive self-renewal capacity and are competent to differentiate into any cell type of the body. They are valuabl... L Gerrard,L Rodgers,W Cui - 《Stem Cells》 被引量: 491发表: 2010年 Differentiation of Human Embryonic Stem Cells to Regional Specifi...
molecular events controlling differentiation into the earlygerm layersand more distal branches of the developmentaltree. Over the last 15 years an increasing number of groups have become interested in the use of mouse embryonic stem cells as a cell source to treat murine models of cell deficiency....
Currently, there are difficulties associated with the culturing of pluripotent human embryonic stem cells (hESCs), and knowledge regarding their regulatory mechanisms is limited. MicroRNAs (miRNAs) regulate gene expression and have critical functions in stem cell self-renewal and differentiation. Moreover...
Differentiation and isolation of hepatic-like cells from human embryonic stem cells. Human embryonic stem cells are pluripotent cells that can serve as a cell source for transplantation medicine, and as a tool to study human embryogenesis. ... N Lavon,O Yanuka,N Benvenisty - 《Differentiation...
Cell DifferentiationEmbryo, MammalianModels, BiologicalStem CellsIt is widely anticipated that human embryonic stem (ES) cells will serve as an experimental ... Pera,F M. - 《Development》 被引量: 1167发表: 2004年 Enrichment of human embryonic stem cell-derived NKX6.1-expressing pancreatic progenit...
Genetic modification of human embryonic stem cells for derivation of target cells. Directed differentiation of human embryonic stem cells (hESCs) may yield models to study organogenesis, produce cells and tissues for therapies, and identi... A Giudice,A Trounson - 《Cell Stem Cell》 被引量: ...