The NS1 gene segments from MR766 virus (Rhesus/1947/Uganda Accession: NC_012432) and PRVABC59 virus (2015/Puerto Rico Accession: KU501215) were human codon optimized using Integrated DNA Technologies Codon Optimization Tool (http://www.idtdna.com/CodonOpt) and modified to contain a C-termina...
The invention discloses optimization of an HIV (Human Immunodeficiency Virus)-gag gene inheritance codon and a preparation method and application of an AIDS (Acquired Immune Deficiency Syndrome) vaccine. A gene provided by the invention is a DNA (Deoxyribonucleic Acid) molecule shown as 13th-1515th...
Cells were stained with anti-human B2M-APC or anti-human B2M-PE (Supplementary Table3) in PBS containing 1% BSA, and an Attune NxT flow cytometer with 96-well autosampler (Thermo Fisher) was used for flow cytometry analysis. Ligand expression was confirmed for engineered HEK293T cells using ...
W. Optimization of membrane protein overexpression and purification using GFP fusions. Nat. Methods 3, 303–313 (2006). Article CAS Google Scholar Gat, U., Nekrasova, E., Lancet, D. & Natochin, M. Olfactory receptor proteins. Expression, characterization and partial purification. Eur. J. ...
Table 1 Codon–anticodon pairing in the human mitochondrial genetic code. Full size table Disorders of human mitochondrial translation result in mitochondrial dysfunction with respiratory defects, and often cause severe clinical symptoms6. To date, 751 pathogenic mutations in mtDNA have been reported, of...
Most codons were switched to that of the most frequently used codons found within the protein coding sequences of the human genome, as based on NCBI statistics (http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=9606). Table 1. Primers used for codon optimization of HPV18 E1^E...
Supplementary Table 2 Surface expression and assay validation of Tango constructs (XLSX 20 kb) Supplementary Table 3 Constitutive activity of Tango constructs (XLSX 17 kb) Supplementary Table 4 Screening results of 91 GPCRs at a small compound library (XLSX 689 kb) Supplementary Table 5 Tango assa...
co = codon optimised, ec = eye cup, ret = retina, RPE = retinal pigment epithelium. Asterisks represent significant differences between constructs analysed by one-way ANOVA with Tukey’s multiple comparison: * =P < 0.05, ** =P < 0.01, *** =P < 0.001, *** =P...
Using protein-per-mRNA differences among human tissues in codon optimization Xavier Hernandez-Alias Hannah Benisty Martin H. Schaefer Genome Biology(2023) Optimization of differential filtration-based mitochondrial isolation for mitochondrial transplant to cerebral organoids ...
fusion partner bRIL using overlap extension PCR. Five residues (E347–M351) were truncated at the C terminus. A point mutation S2115.48L was introduced into the FPR2 gene by standard QuikChange PCR. The codon-optimized DNA sequence and all primer sequences are shown in Supplementary Table4. ...