The distribution of human cell lines used in research should not be hindered by restrictions from donors.Common consentTestis
Cell lines are valuable resources as model for human biology and translational medicine. It is thus important to explore the concordance between the expression in various cell lines vis-à-vis human native and disease tissues. In this study, we investiga
Cell lines used in this study Retinal pigment epithelial cells (hTERT RPE-1, ATCC, CRL-4000) were cultured in DMEM (Gibco, 11995-065) supplemented with 10% fetal bovine serum (FBS; Sigma, TMS-013-B), 2 mM L-glutamine (ThermoFisher Scientific, 25030081) and penicillin/streptomycin (P/S...
and A.N. established patient iPS cell lines used in this study and performed quality control of iPS cells; M.O. helped with FACS data analysis; L.G. and S.I. performed exome sequencing and database analysis; T.Y. analysed RNA-seq and RT–qPCR data with the help of S.S.; K.W....
18 This process enables us to study the causes of specific diseases, such as Alzheimer's Disease, ALS or Parkinson's Disease, and then research a variety of treatments for these diseases. If the stem cell lines are created for any...
In the 21st century, cell culture has become an essential technique for proliferating stem cells for cell therapy. Since 2010, 3D cell culture has been attempted to generate tissue for research or transplantation. In 3D cell culture, cells are grown in a 3D scaffold in which cells differentiate...
Back to topof Research involving human participants, their data or biological material Cell lines If human cells are used, authors must declare in the manuscript: what cell lines were used by describing the source of the cell line, including when and from where it was obtained, whether the ce...
Eight human breast cell lines (human mammary epithelial cells, and MCF10A, MCF7, SUM149, SUM159, SUM1315 and MDA.MB.231 cells) were analyzed using flow cytometry for CD44, CD24, and epithelial-specific antigen (ESA) expression. Limiting dilution orthotopic injections were used to evaluate tum...
All cell lines were originally obtained from the American Type Culture Collection (Manassas, VA) and were maintained at 37 °C in a humidified atmosphere consisting of 5% CO2 and 95% air. Western blot analysis The preparation of whole-cell protein lysates and procedures used for the western ...
Human cancer cell lines have long served as tools for cancer research and drug discovery, but the presence and the source of intra-cell-line heterogeneity remain elusive. Here, we perform single-cell RNA-sequencing and ATAC-sequencing on 42 and 39 human cell lines, respectively, to illustrate ...