16 non-cancerous cell lines, and 8 uncategorized cell lines in which the disease types have not yet been classified (marked as “Unknown”; see Supplementary Data1)8,13. The CCLE 2019 cell line database6provides the transcriptomics data of a total of 1,019 cell...
National Institutes of Health (NIH) Stem Cell Information webpage: contains relevant information on current US stem cell policy; NIH Stem Cell Registry with a list of eligible lines for NIH funding. The page also contains public comments on draft NIH human stem cell guidelines that supplement Exe...
Lifeline®’s primary cells come from a wide variety of donors without the accumulated mutations found in immortal cell lines resulting in better outcomes. PRIMARY CELL CHOICES by Donor Demographic With Lifeline®’s high-quality media, you can narrow down Primary Cells by donor demographics to...
We used high-throughput single-cell RNA-seq to generate a cell atlas of the adult human retina. From 55,736 foveal and 29,246 peripheral retinal cells, we identified 58 cell types. We then used the cell atlas to compare fovea with peripheral retina, and human with macaque retinal cell typ...
The production of hiPS cell lines from peripheral blood samples involved the following three steps: erythroblast enrichment, electrotransfection, and iPSC initiation. In the erythroblast enrichment step, the cells were transfected when the erythroblast population exceeded 80% (Fig. 3). Typically, cells...
Primary cardiac cells are difficult to access and maintain in cell culture for extensive experiments; therefore, the human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) became an excellent alternative. This technology enables a production of high number of patient- and disease-...
Samples were given a standard annotation (data series ID: cell class: chip description: replicate no.; Additional file1: Table S1) and ordered by cell type (embryonic stem cells, induced pluripotent stem (iPS) cells, epithelial cells, fibroblasts etc.). The data analysis pipeline used in ...
based on the assumption that the occurrence of sequencing errors is independent of cell barcode and such errors will occur randomly in all cells (Fig.4A and the “Methods” section). We tested our approach on thescmixologysamples where the five cell lines carry distinct variants. The principal...
Cell-cycle gene list Macosko et al., 2015, Tirosh et al., 2016 N/A Contact for Reagent and Resource Sharing Further information and requests for reagents may be directed to, and will be fulfilled by, the Lead Contact, F.T. (tangfuchou@pku.edu.cn). Experimental Model and Subject Details...
all mouse conditions were chosen based on their resemblance to gene expression patterns in human cell lines (TableS1)55,60,61. All SNP and VNTR data were obtained from peer-reviewed sources linking humanPER3to specific variations in sleeping patterns11,25,30. For a full list of experimental ...