Protein-protein interactionCD14 is a multifunctional pattern recognition receptor, which works in concert with several other toll-like receptors and activates downstream immune response. As CD14 polymorphisms have been associated with several diseases, a study of non-synonymous SNPs will be useful in ...
CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an...
On the other hand, LPS alters membrane TLR4 expression by monocytes devoid of membrane CD14 only in the presence of human serum. The effect of serum is blocked by anti-CD14 monoclonal antibodies, which strongly suggests a functional role for soluble CD14/LPS complexes in the interaction with ...
The following antibodies were used for the analysis of decidual immune cells with FACS, intracellular staining, and cell sorting: PerCP/Cy5.5 anti-human CD45 (HI30), Brilliant VioletTM421 anti-human CD56 (HCD56), APC/Cy7 anti-human CD3 (SK7), PE anti-human CD14 Antibody (63D3), FITC ...
Recombinant Human CD14 Protein Cat # 383-CD (3) Citations (25) Recombinant Human CD4 Fc Chimera Avi-tag Protein, CF Cat # AVI10965 Recombinant Mouse CD4 His-tag Protein, CF Cat # 10476-CD (1) Recombinant Human IFN-alpha H2 (alpha 14) Protein Cat # 11145-1 Citations (1) Re...
et al. Human dendritic cell subset 4 (DC4) correlates to a subset of CD14dim/–CD16++ monocytes. J. Allergy Clin. Immunol. 141, 2276–2279 (2018). Article PubMed Google Scholar Collin, M. & Bigley, V. Human dendritic cell subsets: an update. Immunology 154, 3–20 (2018). ...
Human protein-protein interaction network connecting TGFB1, TGFBR1, SMAD7, SKIL, EGR1, PPARG with genes involved into commonly identified biological processes and pathways. The dashed green line indicates the putative transcriptional regulation of SMAD7 by transcription factor EGR1. The darker the red...
Cells were incubated with monoclonal antibodies to human antigens CD14 (FITC), CD34 (PE), CD45 (PerCP Cy-5.5), and CD133 (APC; Myltenyi Biotec), with respective isotype controls IgG2a (FITC), IgG1 (PE), IgG1 (PerCP Cy-5.5), and IgG1 (APC) (Becton Dickinson), at 4°C for ...
Based on the difference in the CD14 and CD16 expression, two subsets of monocytes were identified in human and other mammalian blood. These subsets have different patterns of adhesion molecules and chemokine receptors that suggests the different mode of their interaction with endothelium and tissue tr...
data in CD45+cells. (K) Bar chart of the relative percentage of CD8+T cell subsets derived from mass cytometry data in CD45+cells. (L) Percentage of CD14 monocytes in PBMCs from YA (n= 8) and AA (n= 8) groups. (M) Percentage of CD14 monocytes in CD45+cells from YA (n= 5...