Modern studies have shown that, under normal conditions, the cornea tends to take in fluid, mainly from the aqueous humour and from the small blood vessels at the limbus, but this is counteracted by a pump that expels the fluid as fast as it enters. This pumping action depends on an ad...
Human skin is enormously well supplied withblood vessels; it is pervaded with a tangled, though apparently orderly, mass of arteries, veins, and capillaries. Such a supply of blood, far in excess of the maximum biologic needs of the skin itself, is evidence that the skin is at the service...
Abnormalities of or reductions in GABAergic interneurons are implicated in the pathology of severe neuropsychiatric disorders, for which effective treatments are still elusive. Transplantation of human stem cell-derived interneurons is a promising cell-b
Blood testosterone assay The whole blood of mice was obtained by extracting the eyeball blood under anesthesia and blood anticoagulant was added. The blood samples were centrifuged at 1200 × g for 5 min at 4 °C, and the plasma was collected. Concentrations of testosterone were assayed...
DNA from each clone was fragmented to 200 to 400 bp and labeled with biotin by nick translation using a Bionick kit (GIBCO-BRL, Gaithersburg, MD). Labeled probe (∼500 ng) was hybridized to metaphase spreads prepared from a phytohemagglutinin-stimulated culture of peripheral blood lymphocytes ...
Images were taken using an Olympus Inverted Light Microscope. Scale bar represents 100 µm Full size image Verification of co-expression of both hCD147 and mCD147 in various tissues and blood in hCD147KI.het-NSG To confirm proper expression of both endogenous mouse CD147 and knock-in ...
Human peripheral blood Peripheral blood mononuclear cells were obtained from healthy volunteers in the Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College under institutional ethical approval [ethical approvals: 2018-KY-001]. Method details Cell isolation and sort...
a and b: Lymphatic microvessels labeled by D2-40 immunostaining. c and d: Blood vessels labeled by CD34 immunostaining. White arrows show blood microvessels labeled by monoclonal antibody of CD34 only. Original magnification: a, c:×100; b, d (portal tract): ×400: B: Child A-LC liver...
pathological traits of AD. Following image acquisition with a fluorescence microscope, automated image analysis was done using CellProfiler.cIndependent replication of the basic population structure of microglia. We used a recently published human microglia single-cell RNA sequencing dataset to confirm the...
Arrows point to blood vessels with high expression of FN in the cortex (c) and medulla (m). Scale bar, 50 μm. Staining was repeated twice with similar results. i UMAP and violin plots showing expression of the activin ligand (INHBA) and inhibitor (FST) in human fetal thymus (hFT)...