The sample is heated briefly and, while being observed through a microscope, pyridine under alkaline conditions in the presence of a reducing sugar is added with a pipette. If blood is present, salmon-colored crystals form. The Takayama test is very sensitive, and even very old bloodstains ...
Fig. 9.4 shows scanning electron microscope micrographs of a type of scaffold, the electrospinning (ES)—thermally induced phase separation (TIPS)—polyester-urethane urea (PEUU) scaffold with internal diameter of 1.3 mm. To fabricate ES-TIPS PEUU scaffold, first the internal PEUU tubular scaffold...
staining a smear of the patient's blood with a Wright's stain, allowing the different types of white cells to be clearly seen under the microscope. A technologist then counts a minimum of 100 WBCs and reports each type of white cell as a percentage of the total white blood cells counted...
Since we used secondary antibodies with an attached fluorochrome labeled cells become fluorescent after excitation under the microscope. C17.2 cells were immunocytochemically stained for the neuronal marker 尾-III-tubulin and the glial marker GFAP. Fixed 661W cells were stained for the cone marker G...
Finally, the stained sample was washed with water and visualized with an optical microscope. Glucose-6-phosphate dehydrogenase assay To perform glucose-6-phosphate dehydrogenase activity assay 200 μL of O negative blood sample was directly incubated with 50 μg mL−1 of active enzyme in ...
Bone marrow smears were stained with Wright Giemsa, and immunohistochemical detection was carried out based on specific conditions. The cytoplasm and quantity of the bone marrow were observed under a microscope. The morphological analysis was performed jointly using two senior laboratory physicians....
This expressed epitope can be detected with a solution containing fluorescently-tagged monoclonal antibodies, or other labeled binding materials, that are directed against the gp120 epitope, which solution is admixed with the whole blood sample. 2) Blood cells (target cells) which have been ...
the anti-A monoclonal antibody(MA) and anti-B MA were labeled with the fluorescent,then fluorescent-labeled antibodies(FLA) were bound with corresponding biological material(such as bloodstain) in the optimum condition,finally the ABO blood type of bloodstain was determined under microscope fluore....
(Fig. 5). The new third compartment, between the blood and the aqueous, was labeled the “iris”, signifying that solutes leaked first into the stroma of theciliary bodyand diffused into the iris stroma (Fig. 6). From there, the solutes partitioned into the AH of the anterior chamber ...
(Electron Microscopy Services). After making the smear of whole blood and its separated components (RBCs and WBCs), the cells were fixed and stained using Wright-Giemsa stain for 5 min. The glass slide is then washed in DI water carefully and dried for observation under the microscope. ...