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Primers for the human EP3 receptor and β-actin.Xuxia SuiYanmin LiuQi LiGefei LiuXuhong SongZhongjing SuXiaolan ChangYingbi ZhouBin LiangDongyang Huang
UK) according to the manufacturer’s protocol. qPCR was performed using S100P (sequences F: 5′-TCAAGGTGCTGATGGAGAA-3′ and R: 5′-ACACGATGAACTCACTGAA-3′) and β-actin primers (F: 5′-ATGTACGTTGCTATCCAGGC-3′ and
For qPCR, each reaction consisted of 1.6 ng/µl cDNA and 0.5 µM of each primer specific for beta-actin (5′-TCC ATC ATG AAG TGT GAC GT-3′ and 5′-TAC TCC TGC TTG CTG ATC CAC-3′), Mini-TCRβ reporter (5′-AGT TGG CTT CCC TTT CTC AG-3′ 5′-CTT GGG TGG AGT...
Human endogenous actin (ACTB) related gene primers|人内源性肌动蛋白(ACTB)相关基因引物 基本信息更多信息 中文名称:人内源性肌动蛋白(ACTB)相关基因引物 中文同义词:人内源性肌动蛋白(ACTB)相关基因引物 英文名称:Human endogenous actin (ACTB) related gene primers ...
Data were expressed as number of copies generated from the standard curve method and corrected for the reference gene β-actin or glyceraldehyde-3phosphate dehydrogenase (GAPDH). Primers used for qRT-PCR are listed in the table below. Cells were exposed to 0.5 mM palmitate pre-complexed to ...
Human IGF2BP1 qPCR Primer Pair,即人IGF2BP1 qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR)...
ACSL primers were designed using Primer3 (21). β-actin primers were from Clontech Labs, Inc. (Mountain View, CA). The PCR products were separated by gel electrophoresis in 2% agarose gels and visualized with ethidium bromide. Real-time PCR RNA was treated with RNase Free DNase I (...
The primers for our RT-qPCR work are presented in Table 2. Figure 3 The differentially expressed profile of m6A-mRNAs in immature red blood cells of Hb CS thalassemia (T) and healthy volunteers controls (N) (*P < 0.05.). Relative mRNA expression, as evidenced by qRT-PCR. The qRT-PCR...
To this end, in the NANOGiCasp9-YFP hPSC line, we knocked-in a second drug-inducible kill-switch (herpes simplex virus-derived thymidine kinase (TK))44 and a fluorescent reporter (mPlum) into a constitutively expressed gene (ACTB [BETA-ACTIN]), thus creating a ACTBTK-mPlum knock-in all...