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Primers for the human EP3 receptor and β-actin.Xuxia SuiYanmin LiuQi LiGefei LiuXuhong SongZhongjing SuXiaolan ChangYingbi ZhouBin LiangDongyang Huang
UK) according to the manufacturer’s protocol. qPCR was performed using S100P (sequences F: 5′-TCAAGGTGCTGATGGAGAA-3′ and R: 5′-ACACGATGAACTCACTGAA-3′) and β-actin primers (F: 5′-ATGTACGTTGCTATCCAGGC-3′ and
Human IGF2BP1 qPCR Primer Pair,即人IGF2BP1 qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR)...
The primers for our RT-qPCR work are presented in Table 2. Figure 3 The differentially expressed profile of m6A-mRNAs in immature red blood cells of Hb CS thalassemia (T) and healthy volunteers controls (N) (*P < 0.05.). Relative mRNA expression, as evidenced by qRT-PCR. The qRT-PCR...
Human endogenous actin (ACTB) related gene primers|人内源性肌动蛋白(ACTB)相关基因引物 基本信息更多信息 中文名称:人内源性肌动蛋白(ACTB)相关基因引物 中文同义词:人内源性肌动蛋白(ACTB)相关基因引物 英文名称:Human endogenous actin (ACTB) related gene primers ...
Table 1 Primer sequences used for template generation of the standard curves and real-time PCR amplification of hIL-5R and beta-actin genes Full size tableResults and Discusion Experimental validation A common forward primer and two exon specific reverse primers were used for the quantification of...
All samples were run in triplicate, and β-actin was used as an internal control. The primer sequences were as follows: hTERT, 5′-CAGGTCTTTCTTTTATGTCACG-3′ (forward) and 5′-CTTCAAGTGCTGTCTGATTCC-3′ (reverse), CCL2, 5′-CTTCTGTGCCTGCTGCTCAT-3′ (forward) and 5′-GCTTCTTTGG...
To this end, in the NANOGiCasp9-YFP hPSC line, we knocked-in a second drug-inducible kill-switch (herpes simplex virus-derived thymidine kinase (TK))44 and a fluorescent reporter (mPlum) into a constitutively expressed gene (ACTB [BETA-ACTIN]), thus creating a ACTBTK-mPlum knock-in all...
TGFBR1 and BMPR2 in freshly isolated human spermatogonia and human pachytene spermatocytes pursue to the methods described previously16. The primers of these genes were designed and listed inTable 4. The expression levels of these genes were normalized to ACTB (actin, beta) and calculated using ...