2.1. Cell line and culture conditions A human astrocyte cell line was purchased from the American Type Culture Collection (CRL-8621TM, SVG p12; ATCC, Manassas, VA, USA) and maintained in ASF104 N medium (Ajinomoto, Tokyo, Japan) supplemented with 10% fetal bovine serum (FBS) (Atlas Biolog...
Human GBM cell lines A172, T98G, U251, U87, GSCs (Human glioma stem cell-like cells), and a normal human astrocyte cell line HA1800 were procured from ATCC (VA, USA). A172 cells were grown in RPMI-1640 medium containing 10% fetal bovine serum (FBS) (Ausbian, Australia). T98G, G...
The established human astrocyte cell line SVG p12 (CRL-8621) was obtained from ATCC (Manassas, VA). SVG p12 cells (hereafter referred to as SVG cells) were maintained in Eagle's Minimal Essential Medium (EMEM; ATCC), supplemented with 10% fetal bovine serum (FBS; Gibco-Life Technologies, ...
中文名称:TALL-1 [Human adult T-ALL] cell line急性T淋巴细胞白血病细胞系 英文名称:TALL-1 [Human adult T-ALL] cell line 保存条件: 低温避光 纯度规格: 1x10(6)viable cells/ml 产品类别: 有机化学品 "TALL-1 [Human adult T-ALL] cell line急性T淋巴细胞白血病细胞系 细胞背景资料:该细胞源于一名...
a, UMAP showing human M1 SNARE–seq2 data, labelled by cell subclass and AC cluster (colours). Astro, astrocyte; Car3, CAR3 gene; CT, corticothalamic cell; ET, extratelencephalic cell; IT, intratelencephalic cell; micro, microglia; NP, near-projecting; oligo, oligodendrocyte; OPC, oligodend...
U-373MG细胞类似产品::Astrocyte type I clone细胞、FU-97细胞、DoTc2 4510细胞 OCI-AML5细胞类似产品::JURKAT E-6.1细胞、HCe-8693细胞、GM07404A细胞 NCI-H345细胞类似产品::MOVAS-1细胞、MAC1细胞、K-1735细胞 RPMI6666细胞类似产品::CCRF/CEM-C7细胞、MPP89细胞、DH 82细胞 ...
2C). Simultaneously, there were no DAS observed around the pluripotent stem cell markers (NANOG, POU5F1 (OCT4)) astrocyte marker (GFAP), neuron marker (RBFOX3), or oligodendrocyte marker (MOG)(Figure S2D), indicating the complete differentiation of iPSCs into iMG and confirming their ...
Finally, M4 included a number of terms that were significantly enriched in both astrocyte models including neuronal development, cell to cell contact, and axogenesis. Together, these data show that astrocytes displayed some profound molecular changes, such as the dysregulation of cholesterol biosynthetic...
The ECM may be obtained by culturing cells known to deposit ECM, including cells of mesenchymal or astrocyte origin. Schwann cells can be induced to synthesize ECM when treated with ascorbate and cAMP. See, e.g., Baron-Van Evercooren et al., “Schwann Cell Differentiation in vitro: Extracell...
Preparation of rat astrocyte cDNA library Total RNA was prepared from rat primary astrocytes (1.1×108cells), in which ORP150 had been induced under hypoxic conditions, by the acid guanidinium-phenol-chloroform method Chomczynski, P. and Sacchi, N., Anal. Biochem., 162, 156-159 (1987)!. ...