优选的是,所述步骤1)包括:选取htlv-1的gp46、gp21,htlv-2的gp46三个基因和通过linker将htlv-1的gp46、gp21与htlv-2的gp46三个基因连接形成的一个htlv-1/2嵌合基因作为目的基因,再将上述4个基因进行合成,分别插入到原核表达质粒中,分别获得4个重组原核表达质粒,然后再将含htlv-1的gp46、gp21基因,含htlv-2...
The aim of this study was to evaluate the analytical importance of gp21 reactivity and evaluate if isolated gp21 reactivity could be reported as negative. Among the samples that were analyzed 25 were negative, three were indeterminate with gp21 reactivity and one was invalid. Out ...
已有的htlv-1分型方法大都基于ltr、gp21或部分gp46基因,capdepont等利用94株env(gp46+gp21)全长序列所做的同源性分析发现,通过对env全长序列的同源性比对进行htlv-1基因分型,比用ltr的序列要更细致准确,而gp46区域集中了env基因全长差异位点中的65.9%。 现有检测该病毒的方法主要有: 1、htlv核酸的检测:核酸的...
《武汉工业学院学报》.2009,第28卷(第4期),第52-54页.牛华 等.HTLV-I gp21外膜蛋白基因片段的克隆和融合表达. 《中国输血杂志》.2001,第14卷(第6期),第337-339页.审查员 周洋 (54)发明名称HTLV-1/2抗体的快速检测方法(57)摘要本发明公开了一种HTLV‑1/2抗体的快速检测方法,包括以下步骤:1)选取目的...
Gp21 peptides derived from HTLV-I and HTLV-II for use in diagnosis. NEW been discovered HTLV-I and HTLV-II PEPTIDES FOR USE IN DIAGNOSTIC TESTS CONFIRM TO DETECT AND HTLV-I and HTLV-II in human serum. RESULTING FROM THE REG... KG Hadlock,CJ Goh,SKH Fong 被引量: 0发表: 2005年 ...
摘要: NEW MATERIAL: This nucleotide sequence capable of coding for at least one epitope from an immunodominant conserved region of HITV-I env. gp21.收藏 引用 批量引用 报错 分享 文库来源 其他来源 求助全文 SYNTHESIZED ENVELOPE PEPTIDE OF HTLV-1 优质文献 ...
In addition, the present invention focuses on a composition of these HTLV p24 antigen and HTLV gp21 antigen, and an immunoassay method for detecting HTLV antibody using the antigen of the present invention. The use of HTLV p24 antigen in in vitro diagnostic assays and reagent kits containing ...
In addition, the invention focuses on compositions of these HTLV p24 antigens with HTLV gp21 antigen and on an immunoassay method for detection of HTLV antibodies using the antigens of the invention. Also the use of HTLV p24 antigens in an in vitro diagnostic assay as well as a reagent ...