1. Prepare PCR reactions as outlined in Table 2 below. For each sample to be run, pipette 10 µL of the eluted DNA and 10 µL of the Master Mix into a PCR tube. Each PCR reaction will have a final volume of 20 µL. 2. An HSV-2 Negative Control (NegC) and an HSV-2 ...
Define HSV-2 infection. HSV-2 infection synonyms, HSV-2 infection pronunciation, HSV-2 infection translation, English dictionary definition of HSV-2 infection. Noun 1. herpes simplex virus - a herpes virus that affects the skin and nervous system herpes
荧光定量PCR检测生殖器单纯疱疹病毒Ⅱ型DNA 目的为了调查单纯疱疹病毒Ⅱ型在男性和女性患者中的感染情况.方法用荧光定量PCR技术对59例男性和40例女性生殖器溃疡和疱疹患者进行HSVⅡDNA检测.结果男性平均年龄35.0... 欧阳耀灵,彭长华,林静,... - 《中国优生与遗传杂志》 被引量: 13发表: 2004年 腺病毒介导 HSV-...
MTT assay showed that the cells activ-ity transfected with HSV-2 LAT ORF2 remarkably higer than the negative control, While there were no such remarkable changes between them and normal cells. DNA ladder showed that there was no DNA fragment. Therefore we concluded that HSV-2 LAT ORF2 gene...
Virus-2,HSV-2)的基因芯片。该芯片以HSV-2DNA聚合酶的高保守区为靶序列,设计HSV-2特异性引物和探针, 通过PCR反应使扩增产物标记上生物素;氨基修饰的探针固定在活化的玻片上,与生物素标记的扩增产物杂交; 利用生物素与链酶亲和素高亲合力的特性,加入纳米金标记的链酶亲和素后形成生物素-链酶亲和素-纳米金生...
Each PCR reaction contained 10 [micro]l DNA, 17 [micro]l GPR PCR Master 1.0, 1 [micro]l ASR target-specific primer, 1 [micro]l ASR target-specific probe, and 1 [micro]l water, except for HSV1/2, which contained 1 [micro]l each of HSV1 primer, HSV2 primer, HSV1 probe, and ...
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One or both UL39 ddPCR assays failed for 9% of genital swab DNA specimens, correlating with low amounts of HSV-2 DNA in a quantitative PCR test. Overall, we obtained unambiguous UL39 genotyping data from 152 (91%) of 167 persons on whom it was attempted. Our final data set (Table...
False-negative type-specific glycoprotein G antibody responses in STI clinic patients with recurrent HSV-1 or HSV-2 DNA positive genital herpes, The Nether... False-negative type-specific glycoprotein G antibody responses in STI clinic patients with recurrent HSV-1 or HSV-2 DNA positive genital ...
DNA previously purified from HSV-1 (McKrae) strain was used as a positive control and DNA previously extracted from an uninfected animal was used as a negative control. HSV-1 specific primers were used to detect HSV-1 (F strain) DNA. The primers and probe detect the HSV-1 gD gene ...