本指导原则适用于利用基于实时荧光PCR(Real-Time PCR)的核酸检测技术,以特定的单纯疱疹病毒1型(Herpes simplex virus type 1,HSV-1)和/或单纯疱疹病毒2型(Herpes simplex virus type 2,HSV-2)基因序列为检测目标,对来源于人体样本中的单纯疱疹病毒DNA进行体外定性检测或分型检测的试剂,临床主要应用于生殖器疱疹中...
DNA was extracted and PCR testing was performed using type-common primers to the HSV glycoprotein B gene, with the first positive swab from each participant confirmed to be HSV-1 using a type-specific probe.18Swabs with at least 2.3 log...
A“negative” viral culture or PCR result could mean you don’t have genital herpes. But in some cases, a person could still have genital herpes and a negative result. That's likely due to other factors related to how much virus there is in the sores. ...
Using PCR routinely would increase detection of HSV, according to Scoular et al, testing a LightCycler real time PCR protocol in a diagnostic laboratory serving a large genitourinary medicine clinic in Glasgow, Scotland. Scoular et al compared performance with routinely used viral culture for ...
1. Prepare PCR reactions as outlined in Table 2 below. For each sample to be run, pipette 10 µL of the eluted DNA and 10 µL of the Master Mix into a PCR tube. Each PCR reaction will have a final volume of 20 µL. 2. An HSV-2 Negative Control (NegC) and an HSV-2 ...
HSV PCR, however, was negative, but given the clinical evidence for herpes simplex encephalitis, acyclovir was started. Over the next week, the patient’s state improved significantly. He became much more alert and oriented and his physical and neurological exam was noted to be normal. His ...
17 HSV-1 Detection DNA was extracted and PCR testing was performed using type-common primers to the HSV glycoprotein B gene, with the first positive swab from each participant confirmed to be HSV-1 using a type-specific probe.18 Swabs with at least 2.3 log10 HSV-1 copies/mL (3 copies...
Analysis of retinal DNA by PCR at 28 days postinjection revealed the presence of vector DNA in three of three samples, indicating that loss of vector did not explain the loss of FGF2 expression. In Vivo Neuroprotection To test the neuroprotective ability of 2526/FGF2 in vivo, we used a ...
DNA was isolated using phenol/chloroform extraction followed by ethanol precipitation. HSV-1 genomes were quantified using quantitative PCR (ABI 7500, Applied Biosystems) using HSV-1 specific primers and Fast SYBR Green Master Mix (Life Technologies). Primers are listed in Supplementary Table 1. ...
Cells lines were passaged within 6 months of receiving from ATCC and characterized with DNA profiling by the cell bank. PCR was used to confirm that the cells were Mycoplasma-free before this study. Construction of oHSVs expressing NIS or firefly luciferase was carried out using HSVQuik ...