Bohannon from the Feinberg School of Medicine at Northwestern University for providing HSV-1 strain Patton encoding the mCherry-UL25 fusion. We thank members of both branches of the Laboratory of Human Genetics of Infectious Diseases for helpful discussions: T. Kochetkov for technical assistance; B...
7倍和21倍(P<0. 001),而Poly[I:C]刺激无明显变化(P>0. 05),表明IFIT1是通过DNA信号通路来行使其负反馈调节作用.为研究IFIT1基因的抗病毒作用,利用CRISPR/Cas9技术改造的HSV-1-VP26-mCherry病毒感染该敲除细胞株,通过测定病毒荧光数及病毒拷贝数,发现IFIT1敲除细胞株与L929 WT细胞相比,存活率提高了60%(P...
研究H129在神经元中的轴突运输规律及机制有助于对病毒工具进一步改造和优化。 该研究首先基于H129-BAC的遗传改造平台,在H129核衣壳蛋白VP26融合表达红色荧光蛋白mCherry,在囊膜糖蛋白gD融合表达绿色荧光蛋白GFP,构建出核衣壳标记红光,囊膜标记绿光的重组病毒H129-G/R。在微流控原代鼠胚脑皮层神经元的培养系统中对...
Vector cell-type affinity in the retina was assessed via co-staining of viral mCherry (mCh) expression and markers of rhodopsin (photoreceptor marker) or retinoid isomerohydrolase (retinal pigment epithelial cell (RPEC) marker). Hematoxylin and eosin staining was used to observe inflammatory cell ...
When the cells had grown to a confluent monolayer in the disc (3 days after seeding), the motor was briefly stopped, a suspension of HSV-1 mCherry in αMEM was filtered (0.4 µm) and added to the medium chamber (multiplicity of infection (MOI) of 6), and the rotation was reinitia...