实验方法 病毒和细胞培养:使用 Vero 猴肾细胞和 Neuro-2A 细胞培养 HSV-1 菌株 McKrae,在特定培养基中培养细胞并维持环境条件。 动物实验:选用 6 - 8 周龄的 C57BL/6 J 小鼠,适应环境后,经眼部感染 105PFU/mL 的 HSV-1 菌株 McKrae。在感染后的不同时间点采集口腔拭子检测病毒。30 天后,认定小鼠进入...
HSV-1 strain McKrae is more neuroinvasive than HSV-1 KOS after corneal or vaginal inoculation in mice. Virus Res. 2013;173:436-440.Wang H, Davido DJ, Morrison LA (2013) HSV-1 strain McKrae is more neuroinvasive than HSV-1 KOS after corneal or vaginal inoculation in mice. Virus Res ...
Mice were infected using corneal scarification using 5 × 105 PFU of HSV-1 McKrae strain. a–c Tissue plaque assay for trigeminal ganglion (left), brainstem (middle), and brain (right) (n = 5 mice per group). Two-tailed Mann–Whitney U test was used where p < 0.05 ind...
DNA previously purified from HSV-1 (McKrae) strain was used as a positive control and DNA previously extracted from an uninfected animal was used as a negative control. HSV-1 specific primers were used to detect HSV-1 (F strain) DNA. The primers and probe detect the HSV-1 gD gene ...
To determine the role of the amino terminus of herpes simplex virus-1 (HSV-1) glycoprotein K (gK) in corneal infection, neuroinvasion, and establishment of virus latency in trigeminal ganglia of mice.The recombinant virus HSV-1 (McKΔgK31-68) was constructed by engineering gK genes encoding...
目的建立HSV-1型病毒性心肌炎体外细胞模型,观察HSV-1感染离体新生大鼠心室肌后对于细胞电生理指标的影响,同时利用药物改变心肌细胞内的基础自吞噬水平及感染后自吞噬水平,观察自吞噬在HSV-1型病毒性心肌炎治疗中的电生理作用.方法单纯疱疹病毒1型(herpes simplex virus type 1,HSV-1,McKrae毒株)经Vero细胞扩增后,以...
Methods: Cultured HCEC and CV-1 cells were exposed to the McKrae strain of HSV-1 at 5 and 0.0001 multiplicities of infection (MOI). Using the 50% tissue culture infectious dose (TCID50) titration method, viral adsorption (at 5 MOI) and total virus production (at 5 and 0.0001 MOI) ...
HSV-1 stocks (McKrae strain, originally obtained from Bryan Gebhardt, LSU Health Sciences Center, New Orleans, LA) were propagated in Vero cells. Stocks were stored at − 80 °C at a concentration of 1 × 10 TLR 9 deficient mice shed more HSV-1 in tears than wild type mice HSV-1 ...
HSV-1 strain McKrae was propagated in green monkey kidney (Vero) cells and maintained at a stock concentration of 1.7 × 108to 1.7 × 109plaque-forming units (PFU)/mL. Anesthetized mice were infected by scarification of the corneal surface with a 25-G 1½” needle and tear...
Compartmentalized and systemic control of tissue immunity by commensals.###HSV-1 strain McKrae is more neuroinvasive than HSV-1 KOS after corneal or vaginal inoculation in mice.###The TvLEGU-1, a legumain-like cysteine proteinase, plays a key role in Trichomonas vaginalis cytoadherence.###Vag...