HSV-1 strain McKrae is more neuroinvasive than HSV-1 KOS after corneal or vaginal inoculation in mice. Virus Res. 2013;173:436-440.Wang H, Davido DJ, Morrison LA (2013) HSV-1 strain McKrae is more neuroinvasive than HSV-1 KOS after corneal or vaginal inoculation in mice. Virus Res ...
To determine the role of the amino terminus of herpes simplex virus-1 (HSV-1) glycoprotein K (gK) in corneal infection, neuroinvasion, and establishment of virus latency in trigeminal ganglia of mice.The recombinant virus HSV-1 (McKΔgK31-68) was constructed by engineering gK genes encoding...
目的建立HSV-1型病毒性心肌炎体外细胞模型,观察HSV-1感染离体新生大鼠心室肌后对于细胞电生理指标的影响,同时利用药物改变心肌细胞内的基础自吞噬水平及感染后自吞噬水平,观察自吞噬在HSV-1型病毒性心肌炎治疗中的电生理作用.方法单纯疱疹病毒1型(herpes simplex virus type 1,HSV-1,McKrae毒株)经Vero细胞扩增后,以...
DNA previously purified from HSV-1 (McKrae) strain was used as a positive control and DNA previously extracted from an uninfected animal was used as a negative control. HSV-1 specific primers were used to detect HSV-1 (F strain) DNA. The primers and probe detect the HSV-1 gD gene ...
Mice were infected using corneal scarification using 5 × 105 PFU of HSV-1 McKrae strain. a–c Tissue plaque assay for trigeminal ganglion (left), brainstem (middle), and brain (right) (n = 5 mice per group). Two-tailed Mann–Whitney U test was used where p < 0.05 ind...
通过使用HSV-1 Mckrae株在角膜上建立HSK感染的动物模型,应用辣根过氧化物酶免疫组织化学染色技术检测HSV-1抗原在角膜组织内存在的时间,结果发现,在角膜病变消失后5,10... 梁凤岐,董晓光,谢立信 - 《眼科研究》 被引量: 0发表: 1991年 单纯疱疹病毒性角膜炎的临床特点及诊疗思维 目前临床上眼部病毒感染性疾病以...
目的 探讨BALB/c小鼠模型在不同条件下感染HSV-1后的临床表现、病理现象及组织器官中的病毒分布特性,为HSV-1不同毒株感染模式小鼠的研究提供综合的参考指标体系.方法本研究利用HSV-1 17+和McKrae毒株,经滴鼻和角膜途径感染不同周龄的BALB/c小鼠,建立急性感染模式;经皮下和足垫途径感染不同周龄的BALB/c小鼠,建...
type 1 (HSV-1) ocular shedding and recurrent corneal epithelial lesions were assessed following an intravenous injection of cyclophosphamide (75 mg/kg) and 24 hr later an intravenous injection of dexamethasone (4 mg/kg) in 24 eyes of 15 rabbits latently infected with HSV-1 strain McKrae. Samp...
Compartmentalized and systemic control of tissue immunity by commensals.###HSV-1 strain McKrae is more neuroinvasive than HSV-1 KOS after corneal or vaginal inoculation in mice.###The TvLEGU-1, a legumain-like cysteine proteinase, plays a key role in Trichomonas vaginalis cytoadherence.###Vag...
HSV-1 strain McKrae was propagated in green monkey kidney (Vero) cells and maintained at a stock concentration of 1.7 × 108to 1.7 × 109plaque-forming units (PFU)/mL. Anesthetized mice were infected by scarification of the corneal surface with a 25-G 1½” needle and tear...