HSV-1 strain McKrae is more neuroinva- sive than HSV-1 KOS after corneal or vaginal inoculation in mice. Virus Res. 173:436-440.Wang H, Davido DJ, Morrison LA. 2013. HSV-1 strain McKrae is more neuroinvasive tha
HSV-1 strain McKrae was propagated in green monkey kidney (Vero) cells and maintained at a stock concentration of 1.7 × 108to 1.7 × 109plaque-forming units (PFU)/mL. Anesthetized mice were infected by scarification of the corneal surface with a 25-G 1½” needle and tear ...
HSV-1 (strains KOS and McKrae) was grown in Vero cells. The Vero cells used were from the lab stock. The titres of the stocks used were 8–14 Â 109 PFU ml À 1. Titres were determined by plaque assay on Vero cells. Beriglobin was used to neutralize extracellular HSV-1 (CSL ...
实验设计分为三个阶段: 感染期:兔眼接种HSV-1 McKrae株(1×105PFU) 治疗期:感染30天后,PPK疫苗组接受AAV8-PPK1(含IL-2)和PPK2(含IL-15)联合滴眼,对照组用空载体 评估期:监测28天内的角膜病变、泪液病毒载量及T细胞应答 关键发现 临床保护效果 PPK组角膜病变面积显著减小(P<0.005),荧光素染色显示损伤减少...
HSV-1 stocks (McKrae strain, originally obtained from Bryan Gebhardt, LSU Health Sciences Center, New Orleans, LA) were propagated in Vero cells. Stocks were stored at − 80 °C at a concentration of 1 × 10 TLR 9 deficient mice shed more HSV-1 in tears than wild type mice HSV-1 ...
Methods: Cultured HCEC and CV-1 cells were exposed to the McKrae strain of HSV-1 at 5 and 0.0001 multiplicities of infection (MOI). Using the 50% tissue culture infectious dose (TCID50) titration method, viral adsorption (at 5 MOI) and total virus production (at 5 and 0.0001 MOI) ...
免疫组织化学结果亦提示,M3减毒株感染小鼠的肝组织病毒抗原表达和Mckrae感染小鼠存在明显差异.这些结果提示了人为修饰某些病毒蛋白的确具有改变HSV-1毒株致病能力的可能.在第二部分的工作中,我们也发现相比于PBS对照组,M5免疫后28天,56天的小鼠分别感染HSV-1野生型毒株(Mckrae,17+)后不出现任何的感染临床症状.血清...
(Honda,K.,etal.,Nature2005,7434:772-777).Incontrast,thepresentstudydemonstratedthatIRF-3-/-miceare8significantlymoresusceptibletoHSVinfectionviathecornealandintracranialroutes.9Followingcornealinfectionwith2x106pfuofHSV-1strainMcKrae,50%ofwild-type10micesurvived,comparedto10%ofIRF-3-deficientmice....
Mice were infected using corneal scarification using 5 × 105 PFU of HSV-1 McKrae strain. a–c Tissue plaque assay for trigeminal ganglion (left), brainstem (middle), and brain (right) (n = 5 mice per group). Two-tailed Mann–Whitney U test was used where p < 0.05 ind...
DNA previously purified from HSV-1 (McKrae) strain was used as a positive control and DNA previously extracted from an uninfected animal was used as a negative control. HSV-1 specific primers were used to detect HSV-1 (F strain) DNA. The primers and probe detect the HSV-1 gD gene ...