实验方法 病毒和细胞培养:使用 Vero 猴肾细胞和 Neuro-2A 细胞培养 HSV-1 菌株 McKrae,在特定培养基中培养细胞并维持环境条件。 动物实验:选用 6 - 8 周龄的 C57BL/6 J 小鼠,适应环境后,经眼部感染 105 PFU/mL 的 HSV-1 菌株 McKrae。在感染后的不同时间点采集口腔拭子检测病毒。30 天后,认定小鼠进入...
Herpes simplex virus type 1 (HSV-1) ocular shedding and recurrent HSV-1 corneal epithelial lesions were assessed after ocular iontophoresis of 0.1% 6-hydroxydopamine followed by topical ocular instillation of 0.1% Propine in ten rabbits latently infected with HSV-1 strain McKrae. Iontophoresis was...
Mice were infected using corneal scarification using 5 × 105 PFU of HSV-1 McKrae strain. a–c Tissue plaque assay for trigeminal ganglion (left), brainstem (middle), and brain (right) (n = 5 mice per group). Two-tailed Mann–Whitney U test was used where p < 0.05 ind...
DNA previously purified from HSV-1 (McKrae) strain was used as a positive control and DNA previously extracted from an uninfected animal was used as a negative control. HSV-1 specific primers were used to detect HSV-1 (F strain) DNA. The primers and probe detect the HSV-1 gD gene ...
To determine the role of the amino terminus of herpes simplex virus-1 (HSV-1) glycoprotein K (gK) in corneal infection, neuroinvasion, and establishment of virus latency in trigeminal ganglia of mice.The recombinant virus HSV-1 (McKΔgK31-68) was constructed by engineering gK genes encoding...
HSV-1 stocks (McKrae strain, originally obtained from Bryan Gebhardt, LSU Health Sciences Center, New Orleans, LA) were propagated in Vero cells. Stocks were stored at − 80 °C at a concentration of 1 × 10 TLR 9 deficient mice shed more HSV-1 in tears than wild type mice HSV-1 ...
Compartmentalized and systemic control of tissue immunity by commensals.###HSV-1 strain McKrae is more neuroinvasive than HSV-1 KOS after corneal or vaginal inoculation in mice.###The TvLEGU-1, a legumain-like cysteine proteinase, plays a key role in Trichomonas vaginalis cytoadherence.###Vag...
Furthermore, the speckles in McIntyre-infected cells were significantly larger than those observed in both 17syn+- and McKrae-infected cells (p = 0.0165 and p < 0.0001, respectively) (Fig. 10G). The impact of HSV-1 on the regulation of gene expression and RNA metabolism was also ...
HSV-1 strain McKrae was propagated in green monkey kidney (Vero) cells and maintained at a stock concentration of 1.7 × 108to 1.7 × 109plaque-forming units (PFU)/mL. Anesthetized mice were infected by scarification of the corneal surface with a 25-G 1½” needle and tear...
type 1 (HSV-1) ocular shedding and recurrent corneal epithelial lesions were assessed following an intravenous injection of cyclophosphamide (75 mg/kg) and 24 hr later an intravenous injection of dexamethasone (4 mg/kg) in 24 eyes of 15 rabbits latently infected with HSV-1 strain McKrae. Samp...