Since the antibody against HSV-2 infection (IgG) provides life-long immunity [35], we fitted I+R to the observed data of the number of sero-positive cases for the estimation of c and ϕ. To estimate c and ϕ, endemic equilibria of I i and R i were solved numerically with varied...
Article posted by Coliseum, September 12th, 2019 Volkspark Stadium – home to Hamburger SV (HSV) in Germany – has installed LiFi in their press center. Journalists are now filing stories using an ingenious means of wireless communication. HSV has become the world’s first football club to us...
cells in green are HSV-1+with DAPI staining in blue. (B) Metamorph quantification of the cornea area positive for HSV-1 antigen. Horizontal bars depict mean of percentage threshold area positive for anti-HSV-1 positive spots ± SEM (*p < 0.05 comparing the two groups as determined...
ELISA plates (Immobilon, Nunc) were coated with anti-human NT-3 pAb (1:500) in carbonate buffer (pH 9.7) and incubated overnight at 4°C (NT-3 ELISA kit; Promega), followed by incubation of samples and detection of NT-3 by using anti-NT-3 mAb (1:4000) and anti-mouse IgG, HRP...
Fast-replicating neurotropic herpesviruses exemplified by herpes simplex virus-1 (HSV-1) naturally infect the central nervous system (CNS). However, most individuals intrinsically suppress the virus during a primary infection and preclude it from signifi
Alexa Fluor 555-conjugated donkey anti-mouse immunoglobulin G (IgG) (1:2,000, Life Technologies) antibody containing 10 μg/mL Hoechst 33342 in PBS (Invitrogen, H3570) was used to visualize the primary antibody and label the nuclei. Cell Viability Assay Vero cells were harvested in a 1.5-...
The efficiency of FLPe-mediated excision of the BAC and stuffer sequences from the integrated HSV-BAC-shuttle DNA was high, with over 99% of recombinant virus harvested after Step2 LacZ-positive and EGFP-negative. In order for recombined virus to be generated, several events need to occur: ...
We pre-treated protein A/G magnetic beads (MCE, NJ) with apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) antibody (Santa Cruz Biotechnology, Dallas, TX) or mouse IgG (CST, Danvers, MA) to form bead–antibody complexes. The vulvar tissues were homogenized ...
The slide was incubated with labeled polymer-HRP anti-mouse IgG antibody (Dako Envision þ System- HRP (DAB) Kit, Cat# K4007) at RT for 30 min and washed in PBS with 0.05% TWEEN 20. DAB þ Substrate-Chromogen solution was applied to cover the specimen and incubated at RT for 2 ...
After washing in PBS, the samples were incubated with anti-mouse IgG secondary antibody Alexa Fluor™ 488 or 594 (1:500 dilution) (Invitrogen, Hercules, CA, USA) for 60 min at 37 °C. The samples were labeled and incubated for 5 min at room temperature with 10 μg/mL of 4′,6-...