calculated via two-way ANOVA with Tukey’s multiple comparisons test.gSurvival analysis of 4T1 tumor-bearing mice after the different treatments (n = 5 mice).hSchematic of the experimental process for lung metastasis model.iTypical images of lung tissues, HE staining images of metastatic foc...
HSV-2 (Herpes simplex virus type 2) is a sexually transmitted virus that causes genital herpes. At present, infection with HSV-2 is lifelong and incurable. An estimated 491 million (13%) people age...
Rabbit polyclonal anti-HA tag Proteintech Cat# 51064-2-AP; RRID: AB_11042321 Mouse monoclonal anti-His tag Proteintech Cat# 66005-1-Ig; RRID: AB_11232599 Mouse polyclonal anti-IgG Proteintech Cat# B900620; RRID: AB_2883054 Rabbit monoclonal anti-N6-Methyladenosine Cell Signaling Technology Cat...
Figure 1. Recruitment and activation of pDCs in inflamed LNs. (A) The frequency (top) and the absolute numbers (bottom) of B220+CD11c+ pDCs in PLNs (rt. PLN) from uninfected (d0) or HSV-infected mice on day 2 (d2). (B) Expression of CD86, CD40, and CD40L on blood pDC precur...
Alexa Fluor 555-conjugated donkey anti-mouse immunoglobulin G (IgG) (1:2,000, Life Technologies) antibody containing 10 μg/mL Hoechst 33342 in PBS (Invitrogen, H3570) was used to visualize the primary antibody and label the nuclei. Cell Viability Assay Vero cells were harvested in a 1.5-...
Figure 2. Hypoxia-regulated expression and activity of HSV-tk (A) Schematic depicting the two promoters used to direct expression of HSV-tk constructs. The cytomegalovirus (CMV) promoter drives the constitutive expression of HSV-tk, thereby serving as the positive control for these experiments. The...
Anti-HSV-2 IgG prevalence among condom users (sexually active) was 52.9%, which was significantly higher than non-condom users (47%) with an O.R 0.75 (0.60–0.93). Figure 3. Positive and negative outcomes for anti-HSV-2 IgG serological test among Indigenous Guarani-Kaiowá, Kadiwéu, ...