Since the antibody against HSV-2 infection (IgG) provides life-long immunity [35], we fitted I+R to the observed data of the number of sero-positive cases for the estimation of c and ϕ. To estimate c and ϕ, endemic equilibria of I i and R i were solved numerically with varied...
cells in green are HSV-1+with DAPI staining in blue. (B) Metamorph quantification of the cornea area positive for HSV-1 antigen. Horizontal bars depict mean of percentage threshold area positive for anti-HSV-1 positive spots ± SEM (*p < 0.05 comparing the two groups as determined...
(k–l) The number of HSV-1-positive cells in six tissue sections from the medulla and pons were quantified, and presented as means ± s.e.m. n ¼ 3–9 per group. (m–o) Organotypic brain slices from WT and Stinggt/gt mice were cultured and infected with 5 Â 103 PFU of ...
Acyclovir, the typical drug used to treat HSV infections, served as the positive control [29]. EGTA, which depleted extracellular Ca2+, as seen in Fig. 4b-d, had similar antiviral effects, indicating that the anti-HSV- 2 effect of blocking the TRPV4 channel is closely related to Ca2+ ...
Patterns of expression differ slightly in the adult: NT-3-specific hybridization was limited only to inner hair cells, which are innervated by TrkC-positive, Type I SGNs (4, 14, 15). Type I SGNs are the primary auditory afferent neurons that deliver sound signal information from inner hair ...
Mouse polyclonal anti-IgG Proteintech Cat# B900620; RRID: AB_2883054 Rabbit monoclonal anti-N6-Methyladenosine Cell Signaling Technology Cat# 56593;RRID: AB_2799515 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076;RRID: AB_330924 Anti-rabbit IgG, HRP-linked Antibody Cell ...
Fast-replicating neurotropic herpesviruses exemplified by herpes simplex virus-1 (HSV-1) naturally infect the central nervous system (CNS). However, most individuals intrinsically suppress the virus during a primary infection and preclude it from signifi
16,17 EpCAM-retargeted RR-oHSV has been shown to enter and spread between hEpCAM-positive cells in a specific manner.17 As a model for non-cancerous cells, we used murine fibroblast 3T6 cells, which is frequently employed to evaluate the infectivity of CR-oHSV for normal cells.18,19,...
Each HSV-1 genome has two repeats of ICP4, which means the SaCas9/gRNA would have two cut sites with one sequence accounting for off-targets, compared to UL8 and UL52 bearing only a single digestion site in the HSV-1 genome (Figure S1C). Also, we have shown that a single SaCas9/...
Segments of the proximal intestine were removed from the same mouse as positive controls for cycling cells. BrdU positive cells were identified by using an BrdU antibody BU-33 (1:250). Antigen expressing cells were detected by the indirect avidin-biotin immunoperoxidase (Vectastain ABC kit, ...