Afterward, the sections were incubated with a secondary biotinylated IgG antibody followed by an anti-biotin-peroxidase reagent for a duration of 20 min. The detection of signals was accomplished using a DAB reagent (Mxb Biotechnologies), and the nuclei were counterstained with hematoxylin. Slide...
We pre-treated protein A/G magnetic beads (MCE, NJ) with apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) antibody (Santa Cruz Biotechnology, Dallas, TX) or mouse IgG (CST, Danvers, MA) to form bead–antibody complexes. The vulvar tissues were homogenized ...
The following antibodies were used: γH2AX (1:1,000; Invitrogen MA12022), HSV-tk (1:400; Invitrogen PA5-67984), Alexa Fluor 488-conjugated goat anti-mouse IgG (1:1,000; Invitrogen A28175), Alexa Fluor 555-conjugated goat anti-mouse IgG (1:1,000; Invitrogen A21422), and Alexa ...
Figure 1. Recruitment and activation of pDCs in inflamed LNs. (A) The frequency (top) and the absolute numbers (bottom) of B220+CD11c+ pDCs in PLNs (rt. PLN) from uninfected (d0) or HSV-infected mice on day 2 (d2). (B) Expression of CD86, CD40, and CD40L on blood pDC precur...
Alexa Fluor 555-conjugated donkey anti-mouse immunoglobulin G (IgG) (1:2,000, Life Technologies) antibody containing 10 μg/mL Hoechst 33342 in PBS (Invitrogen, H3570) was used to visualize the primary antibody and label the nuclei. Cell Viability Assay Vero cells were harvested in a 1.5-...