The invention discloses an HSV1-TK (herpes simplex virus type 1-thymidine kinase) gene recombination oncolytic adenovirus, a preparation method and an application thereof. The genome of the recombination oncolytic adenovirus sequentially comprises a promoter, an adenovirus E1 area gene and an HSV1-...
关键词:HSV 1 -TK 基因;双歧杆菌;原核表达;基因治疗中图分类号:Q786 文献标识码:A 文章编号:1004-311X(2008)06-0011-04Construction of Prokaryotic Expression Plasmid Included HSV 1 -TKGene and Detection of Expressed ProteinWANG Li -qun1, ZHANG Ming-jiang2, MENG Xiang-chen1*(1.Key Laboratory ...
transduction.AfterselectedwithG418 for3 weeks,the HSVl-tk gene Was detectedinthe genomic DNAofthe Tbp—TL cells by PCRtest,withthe primers spicificllydesigned forHSVl・ tk eDNA;and HSVl一tkmRNAwasdetected by using insitu hybradizition method./nvitro experiments demonstrateddose—and time・...
自杀基因(suicide gene)疗法,又称药物敏感基因(drug sensitivity gene)疗法,是生物治疗的主要方法之一,也是目前国外研究较多的方法之一;其中,HSV—TK基因是应用最为广泛,进展最快,且很有前途,最具前景的,典型的自杀基因. 本实验利用质粒G1Na及LNTK具有相同的BamHⅠ和HindⅢ位点成功构建带HSV1—TK基因的逆转录病毒...
HSV-tk gene therapy for human renal cell carcinoma in nude mice[J]. Cancer Gene Ther, 2001, 8(7) : 529 -536.Pulkkanen KJ; Parkkinen JJ; Laukkanen JM; Kettunen MI; Tyynela K; Kauppinen RA; Ala-Opas MY; Yla-Herttuala S.HSV-tk gene therapy for human renal cell carcinoma in nude ...
Key words:HSV-TK gene;gene therapy;retrovirus;bladder cancer;ganciclovir(GCV) 前言:单纯疱疹病毒胸苷激酶(HSV-TK)基因是存在于病毒等原核生物中的基因,是肿瘤基因治疗应用最广泛的自杀基因。它的表达产物,能将一些对哺乳动物细胞无毒性的核苷类似物转化成有毒性的磷酸化物,阻止细胞DNA的正常合成。当HSV-TK基因被...
Split genome-based retroviral replicating vectors achieve efficient gene delivery and therapeutic effect in a human glioblastoma xenograft model In conclusion, these findings suggest that intratumoral delivery of the HSV1-tk gene by the spRRV system is worthy of development in clinical trials for the ...
DHD/K12 cells stably expressing the tk gene were killed in vitro in the presence of low concentrations of ganciclovir, they exhibited a 'bystander effect' when mixed with TK-negative cells. BD-IX rats injected intraperitoneally, either directly or after surgical peritoneal irritations, with DHD/...
Herpes simplex virus-thymidine kinase/ganciclovir (HSV-TK/GCV) gene therapy was performed in five cases of recurrent glioblastoma multiforme. The mean age of cases, three males and two females, was 60 +/- 5 years old. All of the tumors were confirmed pathologically as glioblastoma multiforme an...
1. Methods:By using Adeasy system, recombinant adenoviral plasmid containing KDR promoter-controlled HSV-tk gene (AdKDR-HSV-tk) was constructed. HUVEC+HUVEC/tk混合细胞的存活率,随HUVEC/tk细胞比例的增加而降低,说明腺病毒介导的KDR启动子-单纯疱疹病毒胸苷激酶/丙氧鸟苷系统不但能杀伤tk+细胞,也能杀伤...