We evaluated the molecular mechanism of resistance in herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) gene-transfected murine mammary carcinoma (FM3ATK-/HSV-1 TK+) cells, that were selected for resist
HSV-tk gene therapy for human renal cell carcinoma in nude mice[J]. Cancer Gene Ther, 2001, 8(7) : 529 -536.Pulkkanen KJ; Parkkinen JJ; Laukkanen JM; Kettunen MI; Tyynela K; Kauppinen RA; Ala-Opas MY; Yla-Herttuala S.HSV-tk gene therapy for human renal cell carcinoma in nude ...
The thymidine kinase (tk) gene from Herpes simplex virus type 1 (HSV1) codes for thymidine kinase, an enzyme able to convert nucleoside analogs such as ganciclovir into cytotoxic molecules. InvivoGen has engineered a synthetic CpG-free fusion gene betwee
transducted into human bladder cancer cell T24. HSV-TK gene transfection with GCV can effectively inhibit growth of human bladder cancer cell T24. The results suggest that therapy of HSV-TK gene will be a potential possibility of treatment of human bladder cancer. Key words:HSV-TK gene;gene ...
方法将转HSV-tk基因HTFs(HTFs-tk)与正常HTFs于两种接种密度下以不同比例混合,加入5μg/m l更昔洛韦作用5 d,MTT法检测对HTFs的杀伤作用;染料传输法观察HTFs细胞间隙连接细胞间通讯(G JIC)的能力;免疫细胞化学检测Cx43分布;RT-PCR及W estern b lot检测Cx43表达。 3. Single clone harbouring HSV-TK gene was...
This band corresponds to the predicted molecular weight of HSV1-TK and HSV1-sr39TK, while the control C6 cells had no similar bands. The most widely used gene therapy approach is the transfection of suicide gene, such as the HSV1-TK/GCV system [19-23], in which GCV is first phosphory...
Preparation HSV1-TK probe of the present invention provides, comprising the steps of: (1) with the restriction enzymes HindIII and XhoI of pcDNA3.1 digested vector, linearized; (2) PCR amplification of the C DnaE end, C terminal TK gene, DEVD sequence, the N-terminus of the TK gene, ...
HSV-TKHerpes Simplex Virus Thymidine Kinase Gene Copyright 1988-2018AcronymFinder.com, All rights reserved. Suggest new definition Want to thank TFD for its existence?Tell a friend about us, add a link to this page, or visitthe webmaster's page for free fun content. ...
Key Words Recombinant Adenovirus HSV-TK gene GCV Glioblastoma cell TJ905 人脑胶质瘤是中枢神经系统最常见的恶性肿瘤。目前的常规治疗预后不良。近年来发展起来的基因治疗给胶质瘤的治疗提供了可能。国内已有少数单位构建并在临床应用了携带HSV-TK基因的逆转录病毒载体,该载体的优点是能将病毒基因组永久地插入受其转...
1) HSV-TK gene HSV-TK基因 1. Lung carcinoma targeted expression ofHSV-TK genecontrolled by radioinducible synchronizing positive feedback genetic circuit; 放射诱导HSV-TK基因在肺癌细胞中靶向、高效性表达的研究 2. MethodsHuman Tenon s capsule fibroblasts transferred by HSV-tk gene(HTFs-tk) and norm...