Serum was tested for HSV-specific IgG antibodies at enrollment and at 12, 24, and 52 weeks using the University of Washington Western Blot, which differentiates between HSV-1 and HSV-2 infection.8Samples were considered HSV-1 or HSV-2 po...
Cy3-labeled goat anti-rabbit IgG (Servicebio, Cat# GB21303) and FITC-labeled goat anti-mouse IgG (Servicebio, Cat# GB22301) were used to incubate the sample for 1 h at 25 °C in the dark. The nuclei were then stained with DAPI (Sigma, Cat# D9542). A confocal fluorescence microscope...
IgG >是临床上最常用来检 测 HSV 感染的一种手段 单纯疱疹病毒糖蛋白 G 是抗 HSV 体液免疫应答的主要靶位 I 且使用多 肽作抗原比使用完整的蛋白< gG gD >作抗原有更好 的特异性和抗原性 ZI 因此本研究通过分析 HSV- 的氨基酸序列 用 PCR 技术克隆 HSV-gG 蛋白 中强抗原决定族较集中的基因片段 在大肠...
HSV-1 is an increasing cause of genital herpes, especially in high-income countries that have a declining rate of oral HSV-1 acquisition during childhood,4,5 leaving individuals susceptible at initiation of sexual activity. An “epidemiologic transition” of HSV-1 from oral to genital infection ...
Afterward, the sections were incubated with a secondary biotinylated IgG antibody followed by an anti-biotin-peroxidase reagent for a duration of 20 min. The detection of signals was accomplished using a DAB reagent (Mxb Biotechnologies), and the nuclei were counterstained with hematoxylin. Slide...
Enzyme linked immunosorbent assay (ELISA) was used to determine immunoglobulin G (IgG) antibody titer to the HSV-1 and HSV-2. Results: Totally, HSV-1 and HSV-2 IgG antibody were positive in 285 (79.2%) and 84 (23.3%) subjects, respectively. The seroprevalence of HSV-2 was higher among...
added and included goat anti-rat IgG conjugated with Alexa Fluor 546 (Invitrogen, Eugene, OR), donkey anti-rabbit Alexa Fluor 488 (Jackson ImmunoResearch, West Grove, PA), goat anti-hamster TRITC (Jackson ImmunoResearch) all diluted 1:200 in 0.1% Triton/PBS and incubated overnight at 4 °...
2000, DSX, 手动操作 用途 赛润ELISA classic 单纯疱疹病毒I型IgG/IgM 和II型IgG/IgM或I/II IgG/IgA复合型试剂主要用于定性和/或定量检测人血清或血浆中产生的对抗单纯疱疹病毒I型IgG/IgM 和II型IgG/IgM或I/II IgG/IgA复合型的抗体。试剂可用于急性感染疾病中的鉴别诊断和确认血清转化的病人是否是初期感染,此...
PCR扩增VP22 编码基因.从人PBMC中提取总RNA,RT-PCR扩增hIL-18.VP22,hIL-18按不同需要插入pcDNA3.1-S,构建成3种质 粒:pcDNA3.1-VP22-S,pcDNA3.1-S-IL-18,pcDNA3.1-VP22-S-IL-18.制备DNA壳聚糖微球疫苗,鼻腔 免疫小鼠,同时裸质粒DNA肌注小鼠,ELISA检测小鼠血清IgG,粪便sIgA水平.结果,与pcDNA3.1-S相比,...
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