Serum was tested for HSV-specific IgG antibodies at enrollment and at 12, 24, and 52 weeks using the University of Washington Western Blot, which differentiates between HSV-1 and HSV-2 infection.8Samples were considered HSV-1 or HSV-2 po...
Cy3-labeled goat anti-rabbit IgG (Servicebio, Cat# GB21303) and FITC-labeled goat anti-mouse IgG (Servicebio, Cat# GB22301) were used to incubate the sample for 1 h at 25 °C in the dark. The nuclei were then stained with DAPI (Sigma, Cat# D9542). A confocal fluorescence microscope...
Type specific HSV-1 and HSV-2 assays may help the serological diagnosis of HSV infections, since they allow the correct characterization of the antibody response. Bearing in mind the high specificity of the method for HSV-2 IgG, it might be useful in screening of populations for anti-HSV-2...
HSV-1 is an increasing cause of genital herpes, especially in high-income countries that have a declining rate of oral HSV-1 acquisition during childhood,4,5 leaving individuals susceptible at initiation of sexual activity. An “epidemiologic transition” of HSV-1 from oral to genital infection ...
The antibodies used were mouse anti-GFAP (1:400; Sigma-Aldrich; GA5), Rabbit anti-STING (1:100; IMGENEX, IMG-6485A); and secondary antibodies (1:300), Alexa Fluor 488 Donkey anti-mouse IgG (A21202) and Alexa Fluor 568 Donkey anti-rabbit IgG(A10042) from Life Technologies. RNA ...
high editing indices on oHSV-treated (MOI = 1, 24 h) 4T1 cells after IFNAR-1 blockade.d4T1 cells were infected with oHSV (MOI = 1, 24 h) and IFNAR-1 blockade, the cellular protein was analyzed by western blotting to detect the expression level of ZBP1. Western ...
Fast-replicating neurotropic herpesviruses exemplified by herpes simplex virus-1 (HSV-1) naturally infect the central nervous system (CNS). However, most individuals intrinsically suppress the virus during a primary infection and preclude it from signifi
Immunoglobulin (IgG) tests: Used to detect a longer-lasting antibody, which can help differentiate between a recent infection and a recurrence Certain IgG blood tests can also tell you whether you have HSV-1 or HSV-2. CDC Screening Recommendations ...
added and included goat anti-rat IgG conjugated with Alexa Fluor 546 (Invitrogen, Eugene, OR), donkey anti-rabbit Alexa Fluor 488 (Jackson ImmunoResearch, West Grove, PA), goat anti-hamster TRITC (Jackson ImmunoResearch) all diluted 1:200 in 0.1% Triton/PBS and incubated overnight at 4 °...
HSV 2 IgM Ab Titer 1:20 AB <1:20 (what does this mean?) HSV 1 IgG antibodies >5.00 H <.90 HSV-2 IgG antibodies .16 <.90 Index Values for HSV-1 and HSV-2 antibodies <.90 Negative .90-1.10 Euivocal >1.10 positive So should I assume that the IGM test was a false positive ...