C3 KO mice mortality is elevated following ocular HSV-1 infection. WT and C3 KO male and female mice were infected with 500 PFU (HSV-1)/cornea. (A) WT and C3 KO mice (n = 30/group) were monitored for survival over 14 days pi. *p < 0.05 as determined by Longrank and G...
To further explore the potential role of elevated ZBP1 and NLRP6 expression in oHSV infection in tumor cells, we generated ZBP1-deficient 4T1 and 4MOSC1 cells, as well as NLRP6-deficient 4T1 and 4MOSC1 cells, using CRISPR/Cas9-based gene editing. These cells were then treated with oHS...
MMP-9 was elevated in THP-1 but not in corneal cells. When anti-HSV-IgG(Fab鈥 )2 complexes stimulated THP-1, MMP-9 was reduced to control levels. Pretreatment of THP-1 with anti-TLR-2 and -3 inhibited MMP-9 production. Thus, HSV-IC may stimulate THP-1 through the Fc receptor ...
We assayed immunoglobulin G (IgG) in serum samples from 30 patients and 44 healthy subjects and obtained structural magnetic resonance imaging scans from the same individuals. There were proportionately more patients with elevated ratios than healthy comparison subjects (=3.98, 1 df, P=0.046) and ...
Goat IgG Anti-IP-10 P< CD4+ T cells 38 ± 4b 60 ± 5 14 ± 2 0.05 CXCR3+ cells 3± 2 13 ± 3 3± 2 0.0001 Neutrophils 87 ± 6 115 ± 6 131 ± 8 NS a Ear pinnae (two per group) of sensitized or naive mice were challenged with 1 × 105 PFU HSV-1 admixed with neut...
This leads to an elevated risk of severe viral complications due to primary infection or reactivation (for example, cytomegalovirus (CMV), adenovirus (ADV), or herpes-simplex-virus (HSV)). CMV is known as a major pathogen after kidney Tx, whereas the importance of ADV and HSV is still ...
International Journal of Molecular Sciences Article Recurrent Herpes Simplex Virus Type 1 (HSV-1) Infection Modulates Neuronal Aging Marks in In Vitro and In Vivo Models Giorgia Napoletani 1 , Virginia Protto 1 , Maria Elena Marcocci 1 , Lucia Nencioni 1 , Anna Teresa Palamara 1,2,† and ...
Quantitative analysis of human endogenous retrovirus-K transcripts in postmortem premotor cortex fails to confirm elevated expression of HERV-K RNA in amyotrophic lateral sclerosis. Acta Neuropathol. Commun. 2019, 7, 45. [Google Scholar] [CrossRef] [PubMed] [Green Version] Garcia-Montojo, M.; ...
HSV-1-directed IgG titers were determined in serum or plasma and the corresponding CSF specimen. An overview of positively detected samples is provided inTable S3. Accordingly, 4/4 SAH patient specimens were positive for HSV-1 IgG in serum, and 2/3 in CSF. In both positive CSF specimens ...
HRP-conjugated goat anti-guinea pig IgG (Abcam, Shanghai, China) was used as the secondary antibody at 1:10,000 dilution, incubated in the plate for 1 h at 37 °C, followed by the development of the plates using a tetramethyl benzidine substrate solution (TMB; Sigma, St. Louis, MO, ...