鲁友明课题组在小鼠（Htr2c-Cre）的海马区（CA1）注射H129-ΔTK-mCherry（HSV-ΔTK）和rAAV1/2-...
HSV-ΔTK-hUbC-tdTomato是在HSV-tdTomato(HSV 1型毒株H129基因组整合一个tdTomato 基因)的基础上敲除胸苷激酶(thymidine kinase, TK)基因而获得。因为TK基因的敲除,HSV-ΔTK-hUbC-tdTomato 的体内跨突触传播能力受到限制。在表达TK的辅助病毒的存在下,HSV-ΔTK-hUbC-tdTomato可以从起始神经元传播至突触后的神...
h. 被GFP清晰标记的单个神经元。 顺行跨单级突触标记神经环路 通过在H129基因组中敲除TK基因构建H129-△TK-tdT,并构建AAV-TK-GFP。在小鼠VPM脑区先后在同一位点注射AAV-TK-GFP 和H129-△TK-tdT,结果如图4所示,清晰标记出了VPM区神经元直接投射的下一级...
However, whether H129 is a rigorous anterograde tracer and undergoes anterograde-only spreading are questions of significant interest. In the present study, we evaluated the retrograde labeling efficiency of H129 using a TK and ICP34.5 dual deleted H129 recombinant (named as H306) which was ...
内容提示: 分类号 学号 M201675955 学校代码 10487 密级 硕士学位论文 嗜神经性疱疹病毒 HSV-1 H129 经眼压感受性三叉神经末梢感染下丘脑 学位申请人:张志丹 学科专业 :眼科学 指导教师 :刘海霞 答辩日期 :2019 年 05 月 万方数据 文档格式:PDF | 页数:47 | 浏览次数:35 | 上传日期:2020-01-25 16:33:...
近日,病毒学国家重点实验室罗敏华课题组在《Nature Communications》发表了题为“An HSV-1-H129 amplicon tracer system for rapid and efficient monosynaptic anterograde neural circuit tracing”的研究论文。该研究基于HSV-1 H129扩增子与H129-dTK-T2进行多轮病毒遗传改造,研发出标记亮度高、实验周期短(5天)、操作...
HSV-1 H129株是一株具有独特顺向跨突触传播特性的I型单纯疱疹病毒临床分离株,作为载体在神经环路示踪领域等有着广泛应用。然而,传统分子生物学手段难以实现对HSV-1在全基因组水平的编辑,限制了其进一步应用。研究团队以神经示踪病毒H129-G4为模板,在酵母中合成了带绿色荧光基因的病毒全基因组,将其转染哺乳动物细胞...
They have been developed from herpes simplex virus 1 (HSV-1) strain H129 (H129), and have been successfully applied to map diverse neural circuits. Initially, the anterograde polysynaptic tracer H129-G4 was used by many groups. We then developed the first monosynaptic tracer, H129-dTK-tdT...
近日,中国科学院武汉病毒研究所/生物安全大科学研究中心罗敏华课题组在嗜神经病毒HSV1-H129(H129)的轴突运输规律及机制研究方面取得新进展。该研究发现H129病毒颗粒在轴突中的运输规律主要以核衣壳和囊膜单独的范式、由马达蛋白Kinesin-1驱动的顺向运输,核衣壳与囊膜最后在突触形成的位点,包括轴突末端或轴突曲张体中...
H129using a TK and ICP34.5 dual deleted H129 recombinant (named as H306) which was replication-def i cient innon-dividing postmitotic neurons. The novel tracer was tested in vitro and in vivo for evaluating its invasionproperties and tracing capacities. The results demonstrated that H306 ...