作者为了证实LIG1是hsa_circ_0007919的下游靶点，通过干扰LIG1的表达发现会影响PDAC细胞增殖减少，但细胞凋亡和DNA损伤增加，同时过表达LIG1也会回补hsa_circ_0007919被干扰后影响的细胞增殖、凋亡和DNA损伤现象。这些结果表明，hsa_circ_0007919通过增加LIG1的表达，促进细胞增殖，减少细胞凋亡和DNA损伤。图5. LIG1回补...
作者为了研究hsa_circ_0007919如何调控LIG1的表达,通过FISH/核质分离实验发现hsa_circ_0007919主要分布在细胞核中,利用circAtlas和SPP数据库绘制了韦恩图确定了FOXA1与hsa_circ_0007919结合及LIG1启动子结合的最显著的蛋白,并进一步利用STRING数据库预测了FOXA1的互作蛋白TET1。在前人研究中报道FOXA1可作为多种癌症中的...
作者为了研究hsa_circ_0007919如何调控LIG1的表达,通过FISH/核质分离实验发现hsa_circ_0007919主要分布在细胞核中,利用circAtlas和SPP数据库绘制了韦恩图确定了FOXA1与hsa_circ_0007919结合及LIG1启动子结合的最显著的蛋白,并进一步利用STRING数据库预测了FOXA1的互作蛋白TET1。在前人研究中报道FOXA1可作为多种癌症中的...
④ 总之,hsa_circ_0007919以一种依赖LIG1的方式增强DNA损伤修复以维持细胞活性,从而提高GEM耐药性。靶向hsa_circ_0007919和DNA损伤修复途径可能是PDAC的一种治疗策略。 FISH分析hsa_circ_0007919的细胞定位 疾病研究;胰腺导管腺癌,吉西他滨,生存期,DNA损伤,空间转录组;Lei Xu, Xiao Ma, Xiuzhong Zhang, Zeqiang Re...
为了证实LIG1是hsa_circ_0007919的下游靶点,我们研究了LIG1在GEM耐药PDAC细胞中的作用,发现沉默LIG1导致增殖减少,细胞凋亡和DNA损伤增加(图5A-5G)。此外,我们进一步在hsa_circ_0007919沉默稳定的PANC-1/GEM和PANC-1/GEM细胞中过表达LIG1,发现LIG1过表达逆转了hsa_circ_0007919沉默影响的细胞增殖、凋亡和DNA损伤(图...
hsa_circ_0007919 can promote the metastasis of PC by inhibiting THBS1 expression. hsa_circ_0007919 may be a potential therapeutic target in PC.doi:10.1096/fj.202302422RRMa, XiaoXu, LeiGong, ShuaiWu, NaiGuo, JiaxuanFeng, XinyuZhao, Mengmeng...
Correction: hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinomaDNA repairPANCREATIC ductGEMCITABINEADENOCARCINOMAThis document is a correction notice for an articl...
Hsa_circ_0007919 inhibits the DNA damage, accumulation of DNA breaks and apoptosis induced by GEM in a LIG1-dependent manner to maintain cell survival. Mechanistically, hsa_circ_0007919 recruits FOXA1 and TET1 to decrease the methylation of the LIG1 promoter and increase its transcription, ...
Hsa_circ_0007919 was reduced persistently after inflammatory treatments, and it was relevant to Mayo endoscopic subscores and the expression of tight junction molecules. Finally, hsa_circ_0007919 could harbor miR-138, and let-7a to regulate the targeted mRNAs EPC1 and VIPR1. Several circRNAs ...