作者为了证实LIG1是hsa_circ_0007919的下游靶点，通过干扰LIG1的表达发现会影响PDAC细胞增殖减少，但细胞凋亡和DNA损伤增加，同时过表达LIG1也会回补hsa_circ_0007919被干扰后影响的细胞增殖、凋亡和DNA损伤现象。这些结果表明，hsa_circ_0007919通过增加LIG1的表达，促进细胞增殖，减少细胞凋亡和DNA损伤。图5. LIG1回补...
作者为了研究hsa_circ_0007919如何调控LIG1的表达,通过FISH/核质分离实验发现hsa_circ_0007919主要分布在细胞核中,利用circAtlas和SPP数据库绘制了韦恩图确定了FOXA1与hsa_circ_0007919结合及LIG1启动子结合的最显著的蛋白,并进一步利用STRING数据库预测了FOXA1的互作蛋白TET1。在前人研究中报道FOXA1可作为多种癌症中的...
作者为了研究hsa_circ_0007919如何调控LIG1的表达,通过FISH/核质分离实验发现hsa_circ_0007919主要分布在细胞核中,利用circAtlas和SPP数据库绘制了韦恩图确定了FOXA1与hsa_circ_0007919结合及LIG1启动子结合的最显著的蛋白,并进一步利用STRING数据库预测了FOXA1的互作蛋白TET1。在前人研究中报道FOXA1可作为多种癌症中的...
我们再次在这两种GEM耐药细胞系中沉默hsa_circ_0007919,并在GEM处理的正常PANC-1和CFPAC-1细胞中过表达hsa_circ_0007919 (图2C-2D)。CCK-8实验、FCM实验和DNA Ladder实验结果表明,沉默hsa_circ_0007919可降低细胞增殖,增加细胞凋亡,而过表达hsa_circ_0007919则相反(图2F-2K)。与凋亡实验结果一致,hsa_circ_000791...
1、hsa_circ_0007919通过与FOXA1/TET1结合诱导LIG1转录从而增强胰腺导管腺癌的DNA损伤反应并提高吉西他滨耐药性 hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma Molecular Cancer; IF...
引言:本文是张老师课题组发表在肿瘤领域Top杂志《Molecular Cancer》上的一篇临床基础科研文章,该项目聚焦Hsa_circ_0007919(以下简称circRNA)在胰腺导管腺癌(PDAC)吉西他滨(GEM)耐药中的分子功能和调控机制研究(图1),为解决临床PDAC的GEM耐药提供潜在诊疗转化靶点。
hsa_circ_0007919 can promote the metastasis of PC by inhibiting THBS1 expression. hsa_circ_0007919 may be a potential therapeutic target in PC.doi:10.1096/fj.202302422RRMa, XiaoXu, LeiGong, ShuaiWu, NaiGuo, JiaxuanFeng, XinyuZhao, Mengmeng...
Correction: hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinomaDNA repairPANCREATIC ductGEMCITABINEADENOCARCINOMAThis document is a correction notice for an articl...
Targeting hsa_circ_0007919 and DNA damage repair pathways could be a therapeutic strategy for PDAC.doi:10.1186/s12943-024-01937-9Lei Xugrid.413389.40000 0004 1758 1622Department of General SurgeryAffiliated Hospital of Xuzhou Medical University Xuzhou ChinaXiao Ma...
Finally, hsa_circ_0007919 could harbor miR-138, and let-7a to regulate the targeted mRNAs EPC1 and VIPR1. Several circRNAs were differentially expressed in UC. Hsa_circ_0007919 is related to clinical characteristics and epithelial integrity by binding to hsa-let-7a, hsa-miR-138 to regulate ...