增殖迁移侵袭目的:探讨circ_0003998调控肝癌细胞增殖,迁移和侵袭的分子作用机制.方法:培养人肝上皮细胞THLE-2和肝癌细胞Huh7,MHCC97,Hep3B,PLC/PRF/5,采用qRT-PCR方法检测细胞中circ_0003998和miR-218-5p表达,在Huh7细胞中敲减circ_0003998和miR-218-5p的表达,采用CCK8实验检测细胞增殖活性,Transwell实验检测细胞...
hsa-miR-654-5p overexpression.Dualluci-ferase reporter gene assay indicated that the predicted target site of BMP2 was repressed directly by hsa-miR-654-5p,but this repression did not occur at the mutant predicted target site of BMP2.Conclusion hsa-miR-654-5p can directly repress the mRNA ...