let-7a-5p inhibitor promoted cell proliferation (P < 0.05) and inhibited cell apoptosis of PDLSCs (P < 0.01). Conclusion: hsa-let-7a-5p can inhibit the proliferation of PDLSCs and promote the apoptosis of PDLSCs.张晔琳马丽娅彭旭晖
目的探讨hsa-Let-7a-5p对局限性硬皮病成纤维细胞(LSFs)纤维化过程的影响及其与转化生长因子-β受体(TGF-βR)和TGF-β/Smad信号通路的关系.方法将携带hsa-Let-7a-5p和shTGF-βR1的慢病毒载体及空白慢病毒载体感染LSFs.CCK-8法和划痕实验检测各组LSFs的增殖和迁移情况.RT-qPCR,免疫荧光和Western blot检测转染的...
_0005519expressioninCD4 + Tcellswasconfirmed inasthmaticpatients(n=65)andhealthysubjects(n=30).Hsa‐let‐7a‐5p,thetar‐ getofhsa_circ_0005519,waspredictedbyonlinealgorithmsandverifiedbya dual‐luciferasereporterassay.Correlationassaysbetweentheexpressionofhsa_ circ_0005519andhsa‐let‐7a‐5p,themRNA...
hsa-let-7d-5p qPCR试剂盒 分类:microRNA qPCR Kits 品牌:BioTNT 货号:hsa-let-7d-5p 原价:￥2730 现价:￥2100.00 节省:￥630 规格:1 kits 说明:hsa-let-7d-5p 特异性茎环引物试剂盒 ￥615产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方式...
我们利用生物信息学技术(http://www.mirbase.org/)预测到可能与hsa-circ-000094结合的miRNA,它们分别是hsa-miR-98-5p、hsa-let-7f-5p、hsa-miR-370-5p、hsa-let-7e-5p、hsa-let-7a-5p。通过双荧光素酶报告基因实验发现hsa-...
MCE 国际站:hsa-let-7g-5p antagomir 产品活性:hsa-let-7g-5p antagomir 是一种经过特殊化学修饰的成熟miRNA的互补单链,全链进行甲氧基修饰,在5'端和3'端分别有2个和4个硫代骨架修饰,并在3'端连接有高亲和性胆固醇修饰。miRNA antagomir通过特异性结合成熟miRNA,阻止miRNA与其靶基因的互补配对,从而抑制miRNA的...
MCE 国际站:hsa-let-7i-5p antagomir 产品活性:hsa-let-7i-5p antagomir 是一种经过特殊化学修饰的成熟miRNA的互补单链,全链进行甲氧基修饰,在5'端和3'端分别有2个和4个硫代骨架修饰,并在3'端连接有高亲和性胆固醇修饰。miRNA antagomir通过特异性结合成熟miRNA,阻止miRNA与其靶基因的互补配对,从而抑制miRNA...
结果:(1)长寿组与年轻组血清hsa-let-7g-5p表达水平显著高于老年组(P<0.05);冠心病组hsa-let-7g-5p表达水平显著低于正常对照组(P<0.05),其ROC曲线结果提示hsa-let-7g-5p表达量在冠心病中具有一定诊断价值(AUC=0.6536,P<0.0001).Spearman相关分析结果显示,在长寿群体总体人群中血清hsa-let-7g-5p表达水平与FBG,...
The AROC for transcript levels of hsa-let-7d-5p was 0.68 (P = 0.014; 95% CI, 0.39-0.88) and it was 0.64 for hsa-let-7g-5p (P = 0.028; 95% CI, 0.27-0.89). The cut-off value for let-7d-5p had 0.82 sensitivity and 0.34 specificity. Moreover, the cut-off value for hsa-...
In this study, we found that the miRNA hsa-let-7c-5p was significantly upregulated in EV71-infected rhabdomyosarcoma cells. The overexpression of hsa-let-7c-5p promoted replication of the virus, and the hsa-let-7c-5p inhibitor suppressed viral replication. Furthermore, hsa-let-7c-5p ...