1.Cultivation Method ①Preparation of 125 mL Bottles: Clean 125 mL glass bottles, adjust the paddle position near the bottom to facilitate aggregate formation. After installation, autoclave and dry for later use (not required for disposable bottles). ②Cell Resuscitation: Resuscitate hPSCs in mTeSR...
Finkbeiner 1, Alex Chitsazan 2, Li Dai 1, Alexandra K. Haugan 1, Kayla M. Eschenbacher 1, Dana L. Jackson 3, Cole Trapnell 3, Olivia Bermingham-McDonogh 1, Ian Glass 4, Thomas A. Reh 1 5Show more Add to Mendeley Share Cite ...
Day 25 mDA neurons were harvested from 3D gels or 2D, seeded as clusters on laminin-coated 12 mm glass coverslips, and cultured for 15 days using differentiation medium as described above. Voltage sensitive dyes were then used to monitor the electrophysiological activity of mDA neurons using...
Conventional 2D culture consisting of culturing cells directly on a rigid substrate (e.g. polystyrene or glass), usually coated with substrates that mimic extra-cellular matrix (ECM) composition and promote cell adhesion has been an extremely valuable tool for more than 100 years to model tissues...