Day 25 mDA neurons were harvested from 3D gels or 2D, seeded as clusters on laminin-coated 12 mm glass coverslips, and cultured for 15 days using differentiation medium as described above. Voltage sensitive dyes were then used to monitor the electrophysiological activity of mDA neurons using...
The patch electrodes were made from borosilicate glass capillaries (B-120-69-15, Sutter Instruments) with a resistance in the range of 5–7 MΩ. The pipettes were tip-filled with internal solution containing 125 mM K-gluconate, 15 mM KCl, 10 mM HEPES, 4 mM MgCl2, 4 mM Na2ATP, 0.3 ...