UV(280 nm)用于检测生物源性杂质整体,荧光(FL; Ex 280 nm / Em 348 nm)主要用于检测蛋白质(以Trp残基为指标),MALS散射(LS)特别对纳米粒子类别的大型目标产生高灵敏度的响应。此外,通过MALS可以得到目标的旋转半径(RMS radius)的估计值。 根据MALS的信息,大约13分钟附近是以外泌体(EV)为主要组分的区域,并且...
- HPLC纯度(反相) - HPLC纯度(分子筛) - SEC-MALS分子量 SEC-MALS原理 SEC:尺寸排阻色谱法;有时称为GPC/GFC,即凝胶渗透色谱法或凝胶过滤色谱法。 GPC/SEC是根据不同的分子具有不同的流体体积而进行分离,而不是根据分子的分子量大小。色谱柱中填充了表面具有很多小孔的填料颗粒。当样品分子随流动相一起流经填...
The Wyatt HPLC CONNECT™ is a complete SEC-MALS solution, providing seamless digital synchronization for improved performance. Achieve full digital synchronization between your HPLC system, Wyatt detectors, and ASTRA™ with HPLC CONNECT™, enhancing efficiency and accuracy by: Reducing user errors ...
SEC-HPLC-MALS and Coomassie blue stained SDS-PAGE gel analysis of aggregated MSP142-FVO.Feng, QianKarine, ReiterYanling, ZhangRichard, L. Shimp JrVu, NguyenJoan, A. AebigKelly, M. RauschDaming, ZhuLynn, LambertGregory, E. D. Mullen...
产品名索引 应用实例 应用实例(相关信息)
- HPLC纯度(分子筛) - SEC-MALS分子量 SEC-MALS原理 SEC:尺寸排阻色谱法;有时称为GPC/GFC,即凝胶渗透色谱法或凝胶过滤色谱法。 GPC/SEC是根据不同的分子具有不同的流体体积而进行分离,而不是根据分子的分子量大小。色谱柱中填充了表面具有很多小孔的填料颗粒。当样品分子随流动相一起流经填料时,尺寸较小的分...
2 SEC-MALS has also been reported as a common method in biosimilarity assessments,3 and in the analysis of heparin molecular weights as a qualified method.4 The goal of this application was to evaluate and compare the performance of an industry standard...
2 SEC-MALS has also been reported as a common method in biosimilarity assessments,3 and in the analysis of heparin molecular weights as a qualified method.4 The goal of this application was to evaluate and compare the performance of an industry standard...
UHPLC和SEC-MALS的通用检测 Independent of Chromaphores 不依赖于荧光发色基团,示差折光(dRI)检测器可测量几乎所有溶剂中所有类型样品的浓度。 microOptilab将通用检测功能,无与伦比的浓度范围以及Optilab™熟悉的超高灵敏度和线性度带到了UHPLC领域。
Interaction of sodium caseinate with kappa carrageenan studied by size exclusion -HPLC / MALSCorredig, MVerespej, EDalgleish, D. GBrodkorb, ASPECIAL PUBLICATION- ROYAL SOCIETY OF CHEMISTRY