Chromatographic separation was achieved with C18 column (150mm×4.6mm×5μm), at 25 °C with gradient elution of the mobile phase consisting of methanol–water (2% o -phosphoric acid) at flow rate 1.2mL/min. The analyte was detected at 230nm by UV detector. The retention time of carno...
The concentration of RIS in cerebral tissues of rats was determined by HPLC system with UV detector using berberine hydrochloride as internal standard. The chromatographic conditions were that Wondasil C18 column (250 mm×4.6 mm, 5 μm) was used with mobile phase consisted of acetonitrile-0.01 ...
Because of their relative ease-of use and high sensitivity, UV VIS detectors are the most common type of detector used for high pressure liquid chromatography (HPLC). HPLC UV VIS detectors operate by passing visible and UV light through a sample in a flow cell, then measuring the absorption ...
Refractive Index Detector Refractive index (RI) detection is a cost-effective solution recommended for routine analysis of non-UV absorbing compounds. It is an excellent choice for the analysis of sugars, polymers, surfactants and other compounds that do not contain a chr...
gassed ultrasonically before use. The UV detector was operated at 245 nm. Samples were introduced into the column through a manual injector equipped with a sample loop (20 mL). The flow rate was fixed at 0.9 mL/min at room temperature. ...
2489 UV/Vis Detector The Waters 2489 UV/Visible (UV/Vis) Detector is renowned as the most sensitive and versatile dual-wavelength absorbance detector available for high-performance liquid chromatography (HPLC) and ultra-high-performance liquid chromatography (UHPLC) systems. Wi...
The methodology was then used to evaluate quantitatively the effect on linear range of a shift in detection wavelength or of the detector bandwidth. Although experimental results were globally in accordance with spectroscopic theory, magnitudes observed were rather large. So the linear range could vary...
Acquire UV-Vis spectra in multiple channels over a wide dynamic range. Variable Wavelength Detector (VWD) Analyte must contain a chromophore. Acquire UV-Vis spectra with excellent linearity and noise performance. Fluorescence Detector (FLD) Analyte must contain a fluorophore ...
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The combined use of a dual-UV detector as well as a fluorimetric and a multielectrode electrochemical detector (equipped with a dual electrode, consisting ... A Pappa-Louisi,P Agrafiotou,S Sotiropoulos - 《Methods Mol Biol》 被引量: 3发表: 2012年 Determinacion cuantitativa de aminoacidos ...