Resolve a wide variety of polar and nonpolar analytes with Thermo Scientific™ Acclaim™ PolarAdvantage (PA) columns. These sulfonamide-embedded, reversed-phase columns provide unique selectivity and excellent peak shape for acidic, basic, and neutral analytes. In addition, the mildly hydrophilic su...
Resolve a wide variety of polar and nonpolar analytes with Thermo Scientific™ Acclaim™ PolarAdvantage (PA) columns. These sulfonamide-embedded, reversed-phase columns provide unique selectivity and excellent peak shape for acidic, basic, and neutral analytes. In addition, the mildly hydrophilic su...
(2004) Chromatographic properties of monolithic silica capillary columns for polar and nonpolar compounds in reversed-phase HPLC. Chromatographia 60: pp. S19-S25H. Kobayashi,W. Kajiwara,Y. Inui,T. Hara,K. Hosoya,T. Ikegami,N. Tanaka. Chromatographic Properties of Monolithic Silica Capillary...
A simple, precise, and specific column high-performance liquid chromatographic (HPLC) method with UV absorption diode array and fluorescence detection has been developed by optimizing a previously described method for the simultaneous quantification of 15 polar and nonpolar heterocyclic amines (HAs) in ...
Separate polar, nonpolar, and ionizable compounds in a single analysis and achieve baseline separation of challenging polar analytes without derivatizing using a mixed-mode HPLC column. The stationary phases of Thermo Scientific mixed-mode columns combine reversed phase, ion-exchange, and/or HILIC sel...
The stationary stage is hydrophobic, whereas the versatile stage is a polar liquid, such as water-methanol or acetonitrile mixes. It is based on the hydrophobic collaboration rule, which states that the more nonpolar the substance, the more it will be retained. Size Exclusion...
Normal-phase chromatography, the stationary phase is polar and the mobile phase is nonpolar. In the reversed-phase the stationary phase is nonpolar and the mobile phase is a polar. Flash Column Chromatography (FCC) or Flash Chromatography is the quickest and the easiest way to separate complex ...
4a-diaza-s-indacene (BODIPY FL), 4,4-difluoro-5-(2-thienyl)-4-bora-3a,4a-diaza-s-indacene [BODIPY(558/568)], and dipyrrometheneboron difluoride undecanoic acid (TopFluor) are all metabolized into varying arrays of polar and nonpolar fluorescent lipid products when they are fed to ...
The side-chains of amino acids are generally classified according to their polarity, i.e., nonpolar or hydrophobic vs polar or hydrophilic. Further, the polar side-chains are divided into three main groups: uncharged polar, positively charged, or basic side-chains, and negatively charged or aci...
There is nothing like theMicroSolvCogent Diamond Hydride™ HPLC columns for LC-MS and HPLC. Made from TYPE-C™ silica, they are the perfect choice for very polar and many nonpolar compounds. Used with MS-friendly mobile phases, these columns are routinely employed for metabolites, bioanalysis...