Compound separation. Physical separation of the compounds happens on the column stationary phase. After elution from the column, the separated sample components travel to the detector. Analyte detection. Detection of target analytes based on an electrical signal generated by specific p...
Separations Based on Polarity A molecule’s structure, activity, and physicochemical characteristics are determined by the arrangement of its constituent atoms and the bonds between them. Within a molecule, a specific arrangement of certain atoms that is responsible for special properties and predictable ...
order of relative chromatographic polarity is called an eluotropic series. Mobile phase molecules that compete effectively with analyte molecules for the attractive stationary phase sites displace these analytes, causing them to move faster through the column [weakly ...
When you want to use a charged aerosol detector (CAD) or a mass spectrometer (MS) for your surfactant analysis, select the Acclaim Surfactant Plus LC column. This high-resolution, high-efficiency, high-throughput column has similar selectivity to the Acclaim Surfactant column but has exceptionally...
Low column bleeding for low detector noise Large pore volume for optimal resolution Individual pore sizes for individual molecular weight ranges Multi-pore technology for a broad range of molecular sizes Increased lifetime of GPC/SEC columns by combination of proprietary particle and packing technology...
Except where stated otherwise, all column packings are based on microparticulate silica supports. 2.2.1Size-Exclusion HPLC 1. Column 1:Superdex Peptide HR 10/30 (300\(\times\)10 mm inner diameter [ID]; non-silica-based support; separation range of 100–7000; Pharmacia Biotech, Baie d-Ur...
“Perspectives in Modern HPLC,” a magazine column published four times yearly. I recall my first column contribution was after Pittcon 2013 in Philadelphia. Now 10 years (and 42 articles) later, I revisited Philadelphia on March 19–22, 2023, the first in...
Although the column is the heart of the HPLC instrument and essential to its success, until now no single book has focused on the theory and practice of column technology. 'HPLC Columns' provides thorough, state-of-the-art coverage of HPLC column technology for the practicing technician and ...
Column TypeReversed Phase EndcappedYes PhaseReversed Phase Unit SizeEach Run your prep LC column on the UltiMate 3000 Semi-Preparative HPLC system The workhorse system tailored for robust, safe, and high-performance purifications. Based on proven high-pressure gradient principles, the system takes ...
When you want to use a charged aerosol detector (CAD) or a mass spectrometer (MS) for your surfactant analysis, select the Acclaim Surfactant Plus LC column. This high-resolution, high-efficiency, high-throughput column has similar selectivity to the Acclaim Surfactant column but has exceptionally...