We have also included a short section on hydrophilic interaction chromatography, a technique for the analysis of polar compounds that is drawing interest again in the pharmaceutical industry. Finally, we review what is currently understood about the selectivity of reversed-phase columns....
Acclaim mixed-mode HPLC columns offer excellent selectivity to help you separate polar, nonpolar, and ionizable compounds in a single analysis.
Resolve a wide variety of polar and nonpolar analytes with Thermo Scientific™ Acclaim™ PolarAdvantage (PA) columns. These sulfonamide-embedded, reversed-phase columns provide unique selectivity and excellent peak shape for acidic, basic, and neutral analytes. In addition, the mildly hydrophilic ...
Separate polar, nonpolar, and ionizable compounds in a single analysis and achieve baseline separation of challenging polar analytes without derivatizing using a mixed-mode HPLC column. The stationary phases of Thermo Scientific mixed-mode columns combine reversed phase, ion-exchange, and/or HILIC sel...
Ensure certainty in UPLC, UHPLC, and HPLC analysis tasks with CORTECS Premier Columns, engineered to enhance sample throughput ensuring consistent data accuracy.
SeQuant® ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitte...
The HILIC column provides orthogonal selectivity to the Ascentis Express C18, with the elution typically the opposite of that for C18. The non-polar compounds elute early and the highly polar compounds have greater retention and selectivity.HILIC, 2.7μm Columns 53939-U Ascentis Express HILIC ...
SUPELCOSIL LC-ABZ phase has a unique deactivation technology which provides excellent reversed-phase performance for basic compounds, as well as those that are acidic, polar neutral, and non-polar. Silanol suppressing practices such as using competing amines, operating at high ionic strength, and ad...
Ascentis Express RP-Amide, 2.7μm HPLC Columns While the Ascentis Express C8 and C18 provide classic reversed-phase selectivity, the RP-Amide phase provides alternate reversed-phase selectivity, improved peak shape for bases, 100% aqueous compatibility, and LC-MS compatibility, to our high speed ...
Reversed-phase HPLC (RP-HPLC) on C18-bonded columns eluted with methanol–water mixtures provides the most widely used system. Acetonitrile–water (acetonitrile–buffer) mixtures are more efficient, especially when polar conjugates and/or ecdysonoic acids are present. Adequate systems have been design...