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MethodsThe chromatography was performed on Wondasil C18 column (4.6 mm×250 mm, 5 μm) at the detection wavelength of 254 nm, with acetonitrile (A) -0.2% glacial acetic acid (B) as the mobile phase at the flow rate of 0.8 ml/min. The fingerprint was established through the "TCM chroma...
Suitable for Various Applications: This C18 HPLC Column is suitable for a range of applications, including laboratory research, and can be used with various mechanisms, including SCX/SAX/C18/C8/C4/NH2/HLB. Show more Key attributes Other attributes Place of Origin Shaanxi, China Classification Oth...
A liquid chromatograph (Waters e2695, UV detector 2489, PAD detector, Empower 3 chromatography workstation) equipped with a 254 nm detector and a 4.6 mm  25 cm Shimadzu VP-ODS C18 chromato- graphic column containing a Phenomenex C18 protection column was used. For mobile phase A, sodium...
Buszewski, B., Kowalska, S., Karasov, G., Lehotay, J.: Influence of pH on benzoic acid derivatives’ retention and RP HPLC column classification. J. Liq. Chrom. Rel. Technol. 29 , 2663–2675 (2006)Buszewski, B., Kowalska, S., Karasov, G., Lehotay, J.: Influence of pH on...
Analysis was conducted on a Hypesil Gold column (100 × 2.1 mm, 1.9 μm) with a 17-min linear gradient at a flow rate of 0.2 mL/min. In positive polarity, eluents consisted of 0.1 % Formic Acid in water and Methanol, and in negative polarity, 5 mM ammonium acetate (pH 9.0) and ...
Due to the column types and other various factors, the error between measured retention time (tR) and predicted tR by the relative retention (RR) method cannot be ignored sometimes. In order to improve the reproducibility of chromatographic separation and RR, the method of classification of C18 ...
000gand 0.02 ml aliquots injected in duplicate onto a Bio-Rad HPX 87H ion-exchange column (300 × 7·8 mm) (Bio-Rad Laboratories Ltd, Watford, UK) protected by a 30 mm guard column containing a cation H+ion-exchange resin. The eluting solvent was 0.005 mol l−1H2SO4and the ...
HPLC. Types of HPLC. There are many ways to classify liquid column chromatography. If this classification is based on the nature of the stationary phase and the separation process, three modes can be specified. In adsorption chromatography the stationary phase is an adsorbent (like silica gel or...
Shodex Support Office & Distributors Find your Shodex Distributors. Support Find support for Shodex products and HPLC here. HPLC Lecture Notes The types and characteristics of the separation modes used in HPLC (High Performance Liquid Chromatography) are explained in an easy-to-understand manner. ...