Chromato- graphic classifications of commercially available re- verse-phase HPLC columns. Chromatographia 1997; 44(3-4):151-61.E. Cruz, M. R. Euerby, C. M. Johnson, C. A. Hackett , (1997), Chromatographic classification of commercially available reverse-phase HPLC columns, Chromatographia ...
Columns partitionCluster analysisThe pH role in benzoic acid derivatives chromatographic analysis were under the current study. The retention factor (k), as well as the peak asymmetry (f AS), were determined and compared using five commercially available reversed phase columns. Utilization of ...
There are two groups of HPLC columns: 1, 2 and 3, 4, 5. (Reprinted from Multivariate Pattern Recognition in Chemometrics. 4 Copyright 1992, with permission from Elsevier Science.) of a proposed partitioning of the data. Hence, clusters are defined intuitively, depending on the context of ...
Compounds stick to reverse phase HPLC columns in high aqueous mobile phase and are eluted from RP HPLC columns with high organic mobile phase. In RP HPLC compounds are separated based on their hydrophobic character. Peptides can be separated by running a linear gradient of the organic solvent....
at 37 °C. All steps involving the streptavidin filter tips on the liquid handling robot are fully automated. The sample plate was then removed from the liquid handling robot and acidified to pH 2–3 with formic acid. Peptides were desalted with C18 UltraMicroSpin columns (The Nest Group...
where x is an observation of X, whose dimension is (n×1), and ΣF contains the first F rows and columns of Σ. The threshold for the T2 statistic is given as, T α 2 = F ( m - 1 ) ( m + 1 ) m ( m - F ) F α ( F , m - F ) ( ...
For metabolite quantification, in conventional LC-MS methods, achieving technical reproducibility over an extended period (and between laboratories) is challenging due to issues such as ion suppression, instrument performance drift and aging and contamination of LC columns. We used the UMS chemical isoto...
have been measured for three solutes (benzoic acid, N, N-dimethyl-aniline and Vancomycin) in various chromatographic systems (stationary phases and different mobile phase compositions) with the aim of grouping the systems and studying the possibilities for replacement of columns and eluent compositions...
The classification of chromatographic columns with bonded octadecyl groups in conventional reversed-phase high-performance liquid chromatography (RP-HPLC) and in micellar liquid chromatography (MLC) was completed using unsupervised pattern recognition methods: principal component analysis, cluster analysis, and...
column classificationsilica-based columnsChromatographic separations described in official methods may fail because of the lack of information given in a monograph to characterize a suitable column.At least 600 brands of commercial C18 columns are available on the market and their potentially very ...