Although the column is the heart of the HPLC instrument and essential to its success, until now no single book has focused on the theory and practice of column technology. 'HPLC Columns' provides thorough, state-of-the-art coverage of HPLC column technology for the practicing technician and ...
GLOSSARY OF LIQUID-PHASE SEPARATION TERMS The first glossary of common and not-so-common terms and buzzwords for reference to high performance liquid chromatography (HPLC) columns and column technology was published in 1988 (1). It is time for an update because ? many new terms have arisen ...
Equations, describing the dilution, relative signal enhancement and maximum injection volume as a function of the process parameters are derived and experimentally verified. Theory and experiment are in agreement only if variances of response functions are calculated as their second normalized central ...
In theory, given the smaller particles in the same column length, this column should have higher USP plate count values than the fully porous 5 µm column. Table 2 shows the tabular results of the testing. Table 2. Average system press...
urine and other body fluids. HPLC consists of using a liquid mobile phase to pass under high pressure a mixture of analytes extracted from the sample through a column containing the stationary phase.Analyte separationis based on differences in interaction with both the mobile phase and the stationa...
HPLC法测定龟芪壮骨颗粒中二苯乙烯苷的含量 作者:高永堂1,衣学龙1,王兆华2 【摘要】 目的 应用高效液相色谱法测定龟芪壮骨颗粒中二苯乙烯苷的含量。方法 采用DiamonsilTMC18,乙腈-%磷酸溶液为流动相,流速:1ml/min,检测波长为320nm。结果 线性范围为～μg,r=,平均回收率为%,RDS=%。结论 该方法用于测定龟芪...
Chapter 2 Introduction The VK 8020 provides an easy way to collect samples from a dissolution apparatus and inject them onto an HPLC column. Varian, Inc. Page 16 Introduction VK 8020 Sample Collector / HPLC Autoinjector Station Operator's Manual Revision E, 11/10 P/N 70-9028 The VK 8020 ...
termsandbuzzwordsforreferencetohigh performanceliquidchromatography(HPLC)columnsandcolumntechnologywaspublishedin 1988(1).Itistimeforanupdatebecause •manynewtermshavearisenor,insomeinstances,theiroriginalmeaningshaveexpandedor changed •thevarioustechniquesofcapillaryelectrophoresis(CE)havebecomewelldevelopedandare ...
Analytical Theory Biological, Medical, and Clinical Analysis Biopharmaceutical Perspectives Biopharmaceuticals and Protein Analysis Cannabis Analysis Capillary Electrophoresis Chiral Chromatography ChromAcademy Corporate Profiles Data Acquisition, Handling, and Archiving Data Analysis, Statistics, and Chemometrics Dietary...
were performed using a C18 chromatography column (250 × 4.6 mm, 5 μm, pre-column, Berlin) by applying a mobile phase composed of acetonitrile-phosphate buffer (pH = 2.8, 10 mM), (15:85, v/v). The sample injection volume was 20 µL and the analytical wavelength was 210...