Application of column liquid chromatography (HPLC) to special problems in food chemistry a laboratory noteColumn liquid chromatographyHPLCAmino acidsSugarsBiogenic aminesLiquid chromatography has been used for the determination of amino acids, sugars and biogenic amines in food. Some special problems, for...
https://www.linkedin.com/pulse/what-best-hplc-column-id-analytical-analysis-michael-dong-%E5%94%90%E7%BA%AC%E4%B8%AD?trk=articles_directory What is the best HPLC column i.d. for analytical analysis, and why is everyone still using the 4.6 mm id column? The answer is likely historical ...
Similarly, resolution achieved with a Rapid Resolution 4.6 x 150 mm (3.5 µm) column is equivalent to that of a 4.6 x 250 mm (5 µm) column. High-Throughput Separations Shorter Rapid Resolution columns (15-50 mm) are also the ideal choice when a conventional analytical method is ...
As mentioned above, since the compounds analysed covered a wide range of polarities, different chromatographic conditions were evaluated starting with the selection of the column chemistry. The Synergi-Polar RP column (2.5 μm, 2.1 × 100 mm, Phenomenex), which is an ether-linked phen...
The components of each sample were separated and detected by an HPLC system using a LPG-3400RS quaternary pump, WPS-3000TRS autosampler (maintained at 20°C), TCC-3000RS column oven (maintained at 40°C), Accucore C18 column (150 × 3.0 mm, 2.6 μm particle size), FLD-3100 ...
The RP HPLC analysis of canine meibum was conducted on a Hypersil C18 silica gel column (2.1 3 150 mm, 5 mm particles, ThermoFisher, San Jose, CA) in a n-hexane-acetonitrile-aqueous acetic acid gradient solvent system as described earlier24, 26, 27. In both procedures, the analytes ...
Ascentis Express OH5 is a high-speed, high-performance liquid chromatography column based on Fused-Core®particle technology. The OH5 phase is a highly polar ligand that possesses 5 hydroxyl groups tethered to the silica via novel proprietary linkage phase chemistry. The phase is designed to pro...
On-line micro column preconcentration system based on amino bimodal mesoporous silica nanoparticles as a novel adsorbent for removal and speciation of chromium (III, VI) in environmental samples Article Open access 22 May 2015 Explore related subjects Discover the latest articles, news and stories ...
a, 17-protein digest mixture with 1.32% ACN per minute gradient; b, 17-protein digest mixture with 0.66% ACN per minute gradient; c, separation of the 17-protein digest mixture on narrow-bore column with 1.32% ACN per minute gradient; d, separation of tryptic peptides from deglycosylated ...
nition ability in the analytical separation; 3) increase in the loading capacity in the preparative separation; and 4) long life of the column. We expect that the immobilized CSPs will become more popular and will replace the coated-type CSPs. Acknowledgment. This work has been supported by ...