Figure 1. A magnifying glass is the simplest form of lens used to view objects. Brightfield vs. fluorescence microscopy If you use additional lenses to increase your ability to magnify a sample, you’ve got a compound microscop...
Our study showed that the fluorescence lifetime can be used for a label-free characterization of plastics (microplastics).
IF preparations can be analyzed by various microscopy techniques (e.g. CLSM, Epifluorescence,TIRF,GSDIM), depending on the application or the researcher’s interest. Meanwhile, IF has become indispensable for a large number of research groups which have at least access to a simple fluoresc...
In this edition of HowStuffWorks, we will enter the tiny world of light microscopes and examine the various technologies that let them expose what is otherwise undetectable to the human eye. Contents The Basics Image Quality Types of Microscopy Fluorescence Microscopy The Parts of a Light ...
Single-molecule microscopy has evolved into the ultimate-sensitivity toolkit to study systems from small molecules to living cells, with the prospect of revolutionizing the modern biosciences. Here we survey the current state of the art in single-molecule tools including fluorescence spectroscopy, tethere...
How to optimize TIRF scans.(Microscopy)(Total Internal Ieflectance Fluorescence)(Brief Article)Kramer, Matthias
This would be an excellent assay to implement on a tool like the ImageStream. 3. Measuring autophagy. Historically, autophagy has been defined by the measure of the ‘autophagosome’ by either electron or light microscopy. The marker LC3 could also be used in microscopy techniques, especially si...
Immunofluorescence is a technique that utilizes fluorescence microscopy. It enables the visualization of specific proteins within cells. Validating antibodies through this method makes sure that they are suitable for detecting proteins in their native cellular context which can enhance the reliability of res...
molecule in the sample, leading to fluorescence emission. Two photon microscopy utilises two photons of half the energy (and thus twice the wavelength), that interact with the fluorophore within the timescale of attoseconds of each other, exciting the fluorophore and causing fluorescence emission. ...
Single-molecule microscopy has evolved into the ultimate-sensitivity toolkit to study systems from small molecules to living cells, with the prospect of revolutionizing the modern biosciences. Here we survey the current state of the art in single-molecule tools including fluorescence spectroscopy, tethere...