S3 method for Seurat FindMarkers(object, ident.1 = NULL, ident.2 = NULL, group.by = NULL, subset.ident = NULL, assay = NULL, slot = "data", reduction = NULL, features = NULL, logfc.threshold = 0.25, test.use = "wilcox", min.pct = 0.1, ...
(i) I should integrate all 6 cohorts and subset a pair each time for downstream analysis OR (ii) I should integrate only a pair each time and do DE analysis. And then visualize the genes in integrated object of 6 cohorts Do I expect any feature loss due to multi cohort integration in ...
Ignore this, I need to update the question Convert variables to 1 and 0 How can I write 3 blocks of code to 3 files when the blocks of code and the file names are in different lists Error subset data .txt How to add a counter to each group in dplyr How to add a co...
I add some columns of custom metadata by using the Seurat v3.X command(object$name <- vector),but I can't delete these columns,such as "time","hours".I try many many ways,like subset, subsetdata,and test.tc11$hours >- NULL, all them didn't work. ...
Hey, I am currently working with a SeuratObject from one of our collaborators. This Object is a merged SeuratObject from seven different replicates. I want to unmerge/split the Object into each replicate so I can cluster them together in...