Fixed cell viability staining dyes (amine-reactive dyes) work similar to non-fixed dyes, except that instead of binding to DNA, they bind to proteins. They are added to a sample proceeding fixation, the live cells will bind partially to the dye. This is because the fixed dye ...
Gently rinse your fixed cells with PBS to remove any fixation agent. Be careful (pipette gently!) when applying your PBS so you do not disrupt your fixed cells. When done, your coverslips with their fixed cells can be stored under PBS in the refrigerator for up to three months. 8. Proce...
If you have big cavities you should get them fixed when you can afford it. Otherwise, if you forget your iodine for a couple of weeks the cavities will start to bother you again. I had a dentist remark that I had "many small cavities that have healed over" which surprised him. I did...
Cell proliferation was analyzed and the treatment of live/dead double staining simultaneous fluorescence staining of viable and dead cells was performed to assess the viability on chondrocytes (ATDC-5). Silk fibroin/collagen 70/30 film showed the suitable morphology, physical stability, and biological...
then test the stain to see how it looks. Depending on the test results, you may then choose to thin out the stain, use a different color, or (if you got a close enough match) proceed to apply the stain to the item that you’ve now successfully fixed—cheaply, easily, and possibly ...
The TCR integrates forces in its triggering process upon interaction with pMHC. Force elicits TCR catch-slip bonds with strong pMHCs but slip-only bonds with weak pMHCs. We develop two models and apply them to analyze 55 datasets, demonstrating the model
On the back of your eye is a complex layer of cells known as the retina. The retina reacts to light and conveys that information to the brain. The brain, in turn, translates all that activity into an image. Because the eye is a sphere, the surface of the retina is curved. When you...
When you mix the calcein/AM with the solution bathing the cells, the dye crosses into the cell. Living cells have an enzyme that removes the AM portion, traps the calcein within the cell and allows the calcein to bind calcium so that it fluoresces green under ultraviolet light. Dead cells...
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Store cell (in gelatin) at -20℃ Cell Smear Collect non-adherent cells and wash 2X by cold PBS buffer Re-suspend cells with PBS buffer Add 30-50 µL to settled slide and smear it evenly Air dry slide a little bit and cover cell with 4% paraformaldehyde for 2-4 hours Store cell...