Stromal cells express high amounts of vimentin which can be immunostained with the CLL B-cell receptor Ig014.Mascha, Binder
aCellular properties such as viability can be measured immediately by microscopic evaluation of cells stained with vital dyes such as Trypan blue, or by using flow cytometry and stains such as 7-AAD. 多孔的物产例如生活能力可以立刻测量由细胞的微观评估弄脏与重要染料例如台盼蓝,或者通过使用流程...
cells in lymph node sections stained with H&E, CD3 antibody, and CD45 antibody of a SET-CAN mouse that developed T-cell lymphoma. Neoplastic cells invaded the lymph nodes and stained positive for CD3 but negative for CD45.D:Low- and high-power view of H&E-stained sections of kidney ...
Primary human hepatocytes (PHH) and Hepatic stellate cells (HSC) were co-cultured to produce cell sheets, which were cultured in glucose and lipid containing medium, following which histological and functional analyses were performed. Histological findings showed hepatocyte ballooning, accumulation of ...
The present study examined whether bladder detrusor dysfunction due to partial bladder outlet obstruction (pBOO) could be improved after the treatment of human amniotic fluid stem cells (hAFSCs). 72 female rats were grouped into sham operation, pBOO, and pBOO with hAFSCs treatment (pBOO +...
Stained tissue from the midbrain of a COVID-19 patient shows DNA in the cells' nuceli (blue), dopamine neurons (green) and phosphor-alpha-synuclein (red). Credit: Liuliu Yang A new study reported that SARS-CoV-2, the virus that causes COVID, can infect dopamine neurons in the brain...
Karyology of Cells in Culture This chapter discusses the recently developed trypsin technique to reveal the so-called Giemsa stained banding patterns-G-bands on chromosomes, which is ba... HC Wang,S Fedoroff - 《Tissue Culture》 被引量: 103发表: 1973年 Endoreduplication in Human Fibroblast Cultu...
Cells were then stained for surface markers as above, then fixed and permeabilized with the eBioscience intracellular staining kit as per manufacturer’s instructions, then incubated with combinations of IFNγ APC (BD Bioscience), IL-10 PE (Biolegend), IL-17 eFluor 450 (eBioscience). PBMCs ...
(1:400, Invitrogen, #A11008. RRID: AB_2534072) with excitation and emission wavelengths of 495/525 nm and 578/603 nm respectively. DAPI staining (1:25,000, Thermo Fisher, #62247, excitation 360 nm, emission 460 nm) was performed to visualize nucleated cells. The slides were mounted ...
cells of the trophectoderm in blastocyst-stage preimplantation embryos. For the purpose of viral entry studies, we used fluorescent reporter virions pseudotyped with Spike (S) glycoprotein from SARS-CoV-2, and we observe robust infection of trophectoderm cells. This permissiveness could be attenuated ...